Product Details:
| Minimum Order Quantity | 1 Piece |
| Usage/Application | Laboratory |
| Brand | Himedia |
| Country of Origin | Made in India |
| Recommended Storage Temperature | On receipt store at -20 C |
| Appearance | Pale coloured opaque solution |
| Pack Size | 5vl |
HiMedia's LP Enrichment Supplement 1 (FD214) is a component for the selective differentiation of Listeria monocytogenes from other Listeria species, particularly for use with media like HiCrome™ Listeria Ottaviani-Agosti Agar Base. It contains L-phosphatidylinositol, which is hydrolyzed by the phosphatidylinositol-specific phospholipase C (PIPLC) enzyme produced by L. monocytogenes, forming an opaque halo around colonies to visually differentiate it from other Listeria species, according to the manufacturer's Technical Data Sheet. Intended Use:
- Selective differentiation of Listeria monocytogenes from other Listeria species.
- For use with media that detect PIPLC activity, such as HiCrome™ Listeria Ottaviani-Agosti Agar Base.
Key Components:
- L-phosphatidylinositol: The substrate that is hydrolyzed by L. monocytogenes' PIPLC enzyme.
- Distilled water: The solvent for the active ingredient.
Mechanism of Differentiation:
- Listeria monocytogenes possesses the phosphatidylinositol-specific phospholipase C (PIPLC) enzyme.
- This enzyme hydrolyzes the L-phosphatidylinositol in the culture medium.
- The hydrolysis creates an opaque halo around the L. monocytogenes colonies, allowing for their visual identification.
How to Use (General Procedure):
- Add the sterile contents of one vial of LP Enrichment Supplement 1 (FD214) to a prepared base medium, such as an Ottaviani-Agosti Agar Base, after cooling to a suitable temperature.
- Mix well and pour into sterile Petri plates for use in bacterial culture.
Additional Information:
- Item Code: FD214
- Delivery Time: Ready Stock
- Packaging Details: Box
