Leading Manufacturer of fraser broth base himedia, brilliant green bile broth himedia, lactobacillus mrs broth, granulated (mrs broth, granulated) himedia and bd difc salmonella o antis poly grp a i & vi lyo from Jaipur.
HiMedia's Fraser Broth Base (M1327) is a selective culture medium for the primary and secondary enrichment of Listeria species, particularly Listeria monocytogenes, from food and animal feeds. It provides essential nutrients like peptone and yeast extract for Listeria growth, is formulated to offer optimum growth conditions, and is recommended by organizations like ISO for the detection of Listeria in food products. Key Features and Use
Purpose:Isolation and enumeration of Listeria species from various food samples and animal feeds.
Role:Functions as both a primary and secondary enrichment medium.
Nutritional Content:Contains highly nutritive ingredients such as peptone, tryptone, yeast extract, and HM extract to provide essential nutrients for Listeria growth.
Official Recommendation:Recommended by the ISO standard for the enrichment of Listeria species in food products.
Formulation:Based on the Fraser and Sperber formulation, designed to promote the growth of Listeria.
HiVeg™ Variant:A vegetarian version, the Fraser Secondary Enrichment HiVeg™ Broth Base, is also available, using vegetable-defined peptones to avoid risks associated with animal-derived products.
How It WorksThe medium's composition provides optimal conditions for Listeria to grow while inhibiting the growth of other microorganisms. By using this broth, researchers can enhance the recovery and identification of Listeria from complex samples like food, aiding in their detection and enumeration.
Cream to pale green coloured homogenous free flowing powder
pH
7.00 - 7.40
Pack Size
500g
HiMedia's Brilliant Green Bile Broth is a selective culture medium used for the presumptive identification and confirmation of coliform bacteria, especially in water, wastewater, and food and dairy products. It contains bile and brilliant green to inhibit Gram-positive bacteria, allowing Gram-negative coliforms to grow. The lactose in the broth is fermented by coliforms, producing gas that is detected by an inverted Durham's tube, indicating a positive result. Key Features and Purpose
Selective Medium: It selectively grows coliform bacteria by inhibiting the growth of other microorganisms.
Presumptive Test: It is primarily used for a presumptive test for coliforms.
Inhibitory Components:
Bile (Oxgall): Inhibits Gram-positive bacteria, as well as lactose-fermenting Clostridia.
Brilliant Green: Inhibits Gram-negative bacteria.
Carbohydrate Source: Lactose is provided as a fermentable carbohydrate.
Detection: Gas production from lactose fermentation, detected in an inverted Durham's tube, is a positive indicator of coliform growth.
Applications: Widely used for detecting coliforms in:
Water
Wastewater
Foods
Milk and dairy products
How it Works
Inoculation: Samples suspected of containing coliforms are inoculated into the medium.
Incubation: The inoculated tubes are incubated, usually at 35-37°C for 18-48 hours.
Observation: The presence of gas in the Durham's tube is a presumptive positive result.
Confirmation: Further testing, such as using EC broth at 45°C, may be performed to confirm the presence of faecal coliforms.
Standards
The medium's formulation and performance criteria often adhere to guidelines from organizations like the American Public Health Association (APHA) and the International Organization for Standardization (ISO).
Medium amber, clear to slightly opalescent solution
Packaging type
HDPE
Recommended Storage Temperature
On receipt store between 2-8°C
Specific end uses
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated
pH
6.30 - 6.70
Bulk density
620kg/m³ for the granular form (as per Sigma/Millipore product)
Sterilization
Autoclave at 121°C for 15 minutes (15psi)
Himedia's Lactobacillus MRS Broth, Granulated is a dehydrated, granular culture medium for the isolation and cultivation of all Lactobacillus species. It is a cream to yellow granular medium that, when prepared, forms an amber-colored, clear to slightly opalescent solution. The medium's composition, which includes sodium acetate and ammonium citrate, selectively inhibits the growth of other microorganisms like streptococci and molds. Product features and specifications
Lyophilized powder (to be reconstituted before use)
Shelf Life
1095 days i.e. about ~ years from manufacture
Storage Temperature
2 8 C refrigerated
Tests Per Vial
Sufficient for around 60 tests when rehydrated properly and used as recommended
The BD Difco Salmonella O Antis Poly Grp A I & Vi Lyo is a lyophilized (freeze-dried), polyclonal rabbit antisera used for the serological identification of Salmonella based on their somatic (O) antigens. It contains specific antibodies against the O antigens found in Salmonella serogroups A through I and the Vi antigen, allowing for grouping and identification in a laboratory setting. The product requires refrigeration upon arrival and contains a preservative like Thimerosal (a mercury compound). Key Features
Purpose: To identify and group Salmonella bacteria by targeting specific O-antigens and the Vi-antigen using slide agglutination tests.
