Providing you the best range of macconkey agar w/ cv and w/o nacl, tryptone bile agar, buffered peptone water himedia, listeria identification agar base palcam himedia, sabouraud dextrose agar, granulated himedia and nutrient agar plate himedia with effective & timely delivery.
MacConkey agar with crystal violet (CV) and without sodium chloride (NaCl) is a differential and selective medium used to isolate and differentiate Gram-negative bacteria, particularly enteric pathogens, while inhibiting the growth of Gram-positive bacteria. It's a modification of the original MacConkey agar formulation, where the absence of NaCl and the presence of CV and bile salts restrict the growth of swarming Proteus species and many Gram-positive organisms. The medium differentiates lactose fermenters (e.g., E. coli) from non-lactose fermenters (e.g., Salmonella, Shigella) by observing colony color. Components and Function:
Bile Salts and Crystal Violet:These components inhibit the growth of Gram-positive bacteria, making it selective for Gram-negative bacteria.
NaCl (Absent):The lack of NaCl helps to further restrict the swarming of Proteus species.
Lactose:Lactose is a fermentable sugar that allows differentiation between lactose-fermenting and non-lactose-fermenting bacteria.
Neutral Red:This pH indicator turns red in the presence of acid (produced by lactose fermentation), indicating lactose-fermenting colonies.
How it works:
1. Selective Action:Bile salts and crystal violet inhibit the growth of Gram-positive bacteria, while sodium chloride further restricts swarming Proteus.
2. Differential Action:Lactose is fermented by some bacteria, producing acid. This acid causes the neutral red indicator to turn red, resulting in pink or red colonies.
3. Lactose Non-fermenters:Bacteria that don't ferment lactose will not produce acid, and their colonies will remain colorless or transparent.
Key Characteristics:
Selective: Inhibits Gram-positive bacteria, allowing for the isolation of Gram-negative bacteria.
Differential: Distinguishes lactose-fermenting bacteria from non-lactose fermenters.
Reduces Swarming: The absence of NaCl helps to reduce or prevent swarming of Proteus species.
Cream to yellow coloured homogeneous free flowing powder
pH
7.00 - 7.40
Pack Size
500G
Tryptone Bile Agar (TBA) is a selective culture medium used for the detection and enumeration of Escherichia coli (E. coli) in food, water, and other samples. It is particularly useful for identifying E. coli strains that may be missed by other methods due to their ability to ferment lactose late or not at all. Here's a more detailed description:Composition:
Tryptone (pancreatic digest of casein): Provides essential nutrients for bacterial growth.
Bile salts: Act as a selective agent, inhibiting the growth of Gram-positive bacteria.
Agar: A solidifying agent that forms a gel, allowing for the formation of colonies.
Distilled or deionized water: Used as a solvent to dissolve the other ingredients.
Some formulations may also include other components like sodium chloride or dextrose.
Principle:
TBA is formulated to be selective for Gram-negative bacteria, particularly E. coli, by inhibiting the growth of other bacteria.
It is also designed to be differential, allowing for the identification of E. coli based on its ability to ferment lactose and produce gas.
For example, E. coli colonies may appear pinkish-red with a bile precipitate when grown on Violet Red Bile Agar (VRBA), a related medium.
Use:
TBA is used in the food industry to test for E. coli contamination in raw meats, and other food products.
It is also used in water quality testing and clinical microbiology.
The medium is particularly useful for detecting E. coli strains that are not easily identified by other methods, such as those that ferment lactose late or not at all.
The modified direct plating method (DPM) is often used with TBA to enumerate E. coli.
TBA can also be used with the filter membrane method or other direct inoculation methods.
Variations:
Tryptone Bile Glucuronic Agar (TBX): A variation of TBA that includes a chromogenic substrate (5-bromo-4-chloro-3-indolyl-β-D-glucuronide) to detect the presence of the enzyme β-glucuronidase, which is produced by most E. coli strains. This allows for easy differentiation of E. coli colonies, which appear blue-green on TBX agar, according to HiMedia.
Recommended Storage Temperature : On receipt store between 10-30 DegreeC
On receipt store between 10-30 DegreeC
Appearance
Cream to greenish yellow homogenous free flowing powder
pH
7.80 - 8.20
Himedia's Buffered Peptone Water (BPW) is a non-selective, nutrient-rich pre-enrichment broth designed to recover and resuscitate sub-lethally injured Enterobacteriaceae and other bacteria from food and clinical samples by providing a buffered, inhibitor-free environment. Its composition of proteose peptone, sodium chloride, and a phosphate buffer system supplies nutrients, maintains osmotic balance, and prevents pH changes that could harm stressed microorganisms. It's a recommended medium by international standards like ISO 6579-1:2017 for its role in detecting bacteria such as Salmonella and Cronobacter species prior to selective enrichment. Key Features & Benefits
Resuscitation of Injured Cells:BPW is formulated to enhance the recovery of bacteria that have been damaged by food processing or other environmental factors.
Non-Selective Environment:The medium lacks inhibitory substances, allowing for the growth of a broad range of bacteria.
Buffering System:The phosphate buffer system protects bacteria from detrimental changes in pH, ensuring their viability.
Nutrient-Rich:Proteose peptone provides the necessary carbon, nitrogen, vitamins, and minerals for bacterial growth and repair.
Compliance with Standards:The medium's specifications align with international standards such as ISO 6579-1:2017 for Salmonella detection and ISO 22964 for Enterobacteriaceae from milk products.
Uses
Food Microbiology:It serves as a pre-enrichment medium for detecting Salmonella and other Enterobacteriaceae in food samples.
Clinical Samples:BPW can also be used for the preliminary isolation and recovery of injured bacteria from clinical specimens.
Light yellow to pink coloured homogeneous free flowing powder
Recommended Storage Temperature
On receipt store between 10-30 C
HiMedia's Listeria Identification Agar Base (PALCAM) is a selective culture medium used to isolate and identify Listeria species, particularly Listeria monocytogenes, from clinical and food samples. It contains lithium chloride and polymyxin for selective inhibition of other bacteria, an egg yolk emulsion to aid in the repair of damaged cells, and a dual-indicator system of esculin/ferric citrate and mannitol/phenol red to differentiate Listeria species. Listeria colonies appear grey-green with a black precipitate after incubation, and an overlay with blood can be used to detect hemolysis. Purpose:
Selective Isolation:The agar base is designed to selectively grow Listeria species while inhibiting most other bacteria.
Identification:The dual indicator system helps differentiate between Listeria species and allows for the identification of L. monocytogenes.
Food Safety:It's particularly useful for isolating L. monocytogenes from food products and animal feeds, with performance standards in accordance with ISO 11290-2.
Key Components and Mechanisms:
Selective Agents (e.g., Polymyxin, Acriflavine, Lithium Chloride):These components suppress the growth of other microorganisms, creating a selective environment for Listeria.
Egg Yolk Emulsion:The addition of egg yolk is beneficial for the repair of damaged Listeria cells, which can occur in food samples.
Esculin & Ammonium Ferric Citrate:These react when esculin is hydrolyzed by Listeria, resulting in a black precipitate around the colonies, indicating a positive reaction.
Mannitol & Phenol Red:Listeria species fermenting mannitol produce acidic byproducts, causing a color change of the indicator from red to yellow around the colonies.
Blood Overlay (Optional):Overlaying the inoculated plates with sheep blood helps to identify haemolytic Listeria species by showing a zone of clearing around the colonies.
Growth Characteristics:
Upon incubation at 35-37°C for 24-48 hours, Listeria colonies appear grey-green with a black precipitate, indicating esculin hydrolysis.
When a blood overlay is added, haemolytic Listeria species will show a zone of clearing around the colonies.
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated
Appearance
Cream to yellow coloured granular medium
pH
5.40 - 5.80
HiMedia's Sabouraud Dextrose Agar, Granulated is a microbiology medium used for isolating and cultivating fungi like yeasts and molds, especially those associated with skin infections. Its low pH (around 5.6) and high dextrose concentration inhibit the growth of most bacteria, making it selective for fungi. The granulated form offers ease of handling, dissolution, and storage compared to traditional powdered media. Key features
Selective growth: The medium's acidic pH and high sugar content are specifically designed to suppress bacterial growth, allowing fungi like yeasts, molds, and dermatophytes to thrive.
Wide applications: It is used in both clinical settings and for general microbiology to cultivate fungi from various specimens, including those from skin, and can also be used to test antimycotic substances.
Granulated form: The granulated format ensures quick and complete dissolution, better stability, and is easy to handle and weigh.
Supports aciduric organisms: Besides fungi, it also supports the growth of acid-tolerant (aciduric) microorganisms.
Inhibits bacteria: The low pH (around 5.6) is a key feature that prevents the growth of most bacteria in the sample.
How to use
For isolation from contaminated samples, consider using a selective medium simultaneously with Sabouraud Dextrose Agar.
Incubate cultures for an extended period (up to 4-6 weeks) before reporting them as negative for fungal growth, especially for dermatophytes.
Light Amber coloured medium - Sterile medium in 90mm disposable petri plates
Brand
Himedia
Country of Origin
Made in India
Pack Size
20PT
Recommended storage temperature
20–30 °C
Physical state
solid
pH (value)
7,2–7,6
A HiMedia Nutrient Agar Plate is a sterile, ready-to-use petri dish containing a general-purpose microbiological growth medium. It is used for cultivating a wide variety of non-fastidious microorganisms, for purity checks before further testing, and is also suitable for teaching and demonstrations. The medium typically consists of peptic digest of animal tissue, sodium chloride, beef extract, yeast extract, and agar, with a pH that is generally maintained around 7.2-7.6. Key features
Form: Pre-poured, sterile plates with a solid growth medium.
Purpose: A general-purpose medium for routine cultivation of a wide range of microorganisms, particularly non-fastidious ones.
Common uses:
Maintaining microbial cultures.
Purity checking prior to biochemical or serological testing.
Microbiological examination of water, dairy products, and other materials.
Educational and teaching purposes.
Composition: Contains a balanced mix of nutrients like peptic digest of animal tissue, beef extract, and yeast extract, with sodium chloride to maintain osmotic balance and agar as a solidifying agent.
Storage: Should be stored between 15-25°C.
Enrichment: Can be enriched with additives like serum or blood to cultivate more fastidious organisms.
Sterile medium in 90mm disposable petri plates Yellow to Amber coloured medium
pH
7.30 - 7.70
A HiMedia Luria Bertani (LB) Agar plate is a nutrient-rich medium used for the cultivation of bacteria, especially recombinant strains of E. coli for genetic and molecular biology studies. It contains tryptone (casein enzymic hydrolysate), yeast extract, and sodium chloride to provide essential nutrients, maintain osmotic equilibrium, and support microbial growth. The agar component is what solidifies the medium into a plate for culturing. Key features and uses
Composition: Made with casein enzymic hydrolysate (tryptone), yeast extract, sodium chloride, and agar.
Purpose: Primarily used for cultivating and maintaining recombinant strains of E. coli for genetic and molecular studies. It can also be used for the routine cultivation of other non-fastidious microorganisms.
Nutritional richness: The combination of tryptone and yeast extract provides peptides, peptones, and a Vitamin B complex, while sodium chloride ensures osmotic balance.
Customization: The medium can be modified by adding other substances like antibiotics to select for specific strains or X-Gal for blue-white screening.
Preparation: To prepare, suspend the powder in distilled water, heat to dissolve, sterilize via autoclaving (e.g., at 121°C for 15 minutes), and pour into sterile plates.
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated.
Appearance
Cream to yellow coloured homogeneous free Page 4 of 8 flowing powder
pH
6.80 - 7.20
Tryptone
10.000
HM Peptone B # (equivalent to beef extract)
5.000
Yeast Extract
1.000
Glycine
12.000
Sodium Pyruvate
10.000
Lithium Chloride
5.000
Sulphamethazine (Sulpha)
0.050
Agar
20.000
Final pH (at 25 °C)
7.0 ± 0.2
Physical Form
Powder
Packaging type
HDPE
Selective agent: Contains tellurite and lithium chloride to suppress the growth of most bacteria while allowing Staphylococci to grow.
Growth promoters: Includes pyruvate and glycine to promote the growth of Staphylococci.
Baird Parker Agar Base w/ Sulpha is a selective culture medium used for the isolation and enumeration of coagulase-positive Staphylococcus species, particularly Staphylococcus aureus, from food and other samples. It contains components that inhibit the growth of other microorganisms while promoting the growth of Staphylococci, and it allows for a clear differentiation of Staphylococci colonies based on their metabolic reactions, which include the reduction of tellurite and the breakdown of egg yolk. Key features and components
Differentiation:
Tellurite reduction: Staphylococci reduce tellurite, causing them to appear as grey, black, or brown-grey colonies.
Egg yolk breakdown: Colonies of Staphylococcus aureus are surrounded by an opaque zone due to the breakdown of the egg yolk emulsion, which is often accompanied by a clear halo.
Recommended use:
Used for the isolation and enumeration of coagulase-positive Staphylococci.
Recommended by organizations like AOAC International and ISO for use in food and microbial limit testing.
How to use
Suspend the specified amount of agar base in distilled or de-ionized water.
Heat to dissolve the powder completely.
Autoclave the medium at 121∘C121 raised to the composed with power cap C121∘𝐶 for 15 minutes.
Dispense into sterile containers and allow to cool before adding any required supplements, such as the egg yolk emulsion.
Inoculate the plates with the sample and incubate at the specified temperature.
tellurite Inhibits most Gram-negative and some Gram-positive bacteria; allows S. aureus to
Lithium
chloride Increases selectivity by inhibiting non-target microbes.
Sulphamethazine (Sulpha)
Suppresses swarming Proteus spp. and other competing flora
Optional antibiotics (like polymyxin or novobiocin in some variants)
Further enhances selectivity fo
Addition to Medium
Aseptically add the contents of one vial to 500mL of appropriate sterile, molte
"S Selective Supplement" from Himedia refers to a line of antibacterial supplements, most commonly the S Selective Supplement (FD068) for the selective isolation of Salmonella species. It is a powdered supplement used with a growth medium, such as a base agar, to inhibit the growth of unwanted microorganisms while allowing Salmonella to flourish. These supplements are intended for in vitro diagnostic use by professionals in laboratory settings, particularly for food and clinical sample analysis. Key features of Himedia S Selective Supplement
Purpose: To selectively isolate Salmonella species from a mixed population of bacteria in laboratory cultures.
Composition: A sterile, dehydrated powder, typically containing antibiotics and other selective agents.
Usage: Aseptically rehydrated and added to a sterile, molten growth medium, like a base agar, to create a selective culture plate.
Application: Used for both clinical samples (like pus and wounds) and food samples, following established laboratory guidelines for handling and sterilization of used materials.
Target Organism: Salmonella species.
Other "S" Selective Supplements: Himedia also produces other "S" selective supplements, such as BP S Selective Supplement for suppressing Proteus species and S.F.P. Selective Supplement for Clostridium perfringens.
