Himedia Chemicals
Our product range includes a wide range of sterile disposable square template 25cm himedia, ammonium persulphate hi ar acs, mp 5 medium, pseudomonas agar for fluorescein, antibiotic assay medium no 19 and antibiotic assay medium no 36.
Sterile Disposable Square Template 25cm Himedia
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₹ 1480 / Piece Get Latest Price
| Packaging Size | 1x100NO |
| Grade | microbiology |
| Brand | HiMedia |
| Usage / Application | laboratory |
| Packaging Type | Box |
| Country of Origin | India |
| Model Number | LA1078 |
| Material | Optically clear material for easy reading |
| Disposal | Must be autoclaved at \(121^{\circ }\)C (\(15\) lbs) for \(15\) minutes after use to ensur |
Minimum order quantity: 1 Piece
A HiMedia sterile disposable square template is a sampling aid used for collecting samples in a laboratory, with a size of 25cm225 c m squared25 �2. It is designed for single use to prevent contamination and is part of HiMedia's range of lab consumables and instruments.
Product Name: Sterile Disposable Square Template
Model Number: LA1078
Size: 25cm225 c m squared25 �2
Category: Sampling Aids, under Lab Consumables & Instruments
Features:
Usage: Specifically for collecting samples in a laboratory setting Intended Use / Typical Applications
- Sterile for aseptic use
- Disposable for single use
This kind of template is generally used in microbiological and hygiene monitoring labs — especially for surface sampling (e.g. swabbing, contact plates, cleaning-validation, environmental monitoring, QC in food / pharma / clean-rooms). By placing the template on a surface, you restrict the sampled area to exactly 25 cm² to standardize sampling across samples.
Sometimes similar templates are supplied along with sponge or swab kits for surface hygiene checks. HiMedia Laboratories Pvt. Ltd.+1
Because it’s “sterile” and “disposable,” it helps ensure that sampling is not contaminated by previous use.
Important Notes / Manufacturer Disclaimer
-
As with many lab consumables from HiMedia, this template is intended for laboratory / sampling / in-vitro / environmental-monitoring use — not for use in human or animal therapeutic/diagnostic procedures. Himedialabs+1
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After use with potential contaminants (microbial cultures, environmental swabs, etc.), disposal should follow standard lab bio-hazard disposal protocols (e.g. autoclaving or incineration as per lab guidelines), especially if pathogens or spores are involved. This is consistent with HiMedia’s disposal recommendations for their consumables.
Ammonium persulphate Hi AR ACS
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₹ 760 / Kg Get Latest Price
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| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Product Code | GRM1095 |
Minimum order quantity: 1 Kg
Ammonium persulphate, often labeled "Hi AR" (Hi Media AR or "High AR"), is a white crystalline powder that is highly soluble in water. It's an inorganic compound with the formula (NH4)2S2O8. This reagent is used in various applications like polymer chemistry, as an etchant, and as a cleaning and bleaching agent. Here's a more detailed breakdown:Key Features:
- Appearance: White, crystalline powder.
- Solubility: Highly soluble in water.
- Chemical Formula: (NH4)2S2O8.
- Purity: Hi AR typically indicates a high purity standard, often meeting AR (Analytical Reagent) or ACS (American Chemical Society) standards, which means it's suitable for use in analytical chemistry and other applications where high purity is crucial.
- Applications:
- Polymer Chemistry: Used in the polymerization of various monomers, including aniline, where it acts as an oxidant.
- Etching: Used as an etchant in various materials.
- Cleaning and Bleaching: Used as a cleaning and bleaching agent in various applications.
- Other Uses: It's also used in the preparation of polyacrylamide gels in biochemistry and molecular biology.
- Assay: Minimum 99% by iodometric assay.
- Insoluble matter: Maximum 0.005%.
- Sulphated ash: Maximum 0.05%.
- Acidity (H2SO4): Maximum 0.2%.
- Chloride (CI): Maximum 0.0005%.
- Iron (Fe): Maximum 0.001%.
- Heavy metals (Pb): Maximum 0.001%.
- Oxidizer: Ammonium persulphate is a strong oxidizing agent.
- Irritant: Airborne dust can be irritating to the eyes, nose, throat, lungs, and skin.
MP 5 Medium
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₹ 5510 / Piece Get Latest Price
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| Packaging Size | 500 g |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | Yellow |
| Country of Origin | Made in India |
| Packing Size | 500G |
| Product Code | M596 |
Minimum order quantity: 1 Piece
MP-5 Medium is a microbiological growth medium used to detect pectinolytic microorganisms, particularly those that produce the enzyme polygalacturonase. It's designed to identify organisms capable of degrading pectin, a component of plant cell walls. This is achieved by observing either a clear zone or halo around the microbial colony, indicating pectin degradation, or by using a precipitant solution that reveals zones of pectin hydrolysis. Here's a more detailed breakdown:
- Purpose:MP-5 Medium is specifically formulated to detect pectinolytic activity, which is the ability of microorganisms to break down pectin.
- Pectinase Detection:Many bacteria and fungi produce pectinases (enzymes that degrade pectin). MP-5 Medium helps identify these organisms.
- Detection Method:The medium contains pectin as a substrate. When pectinolytic microorganisms grow on it, they break down the pectin, resulting in a clear zone or halo around the colony. This can be visualized by adding a precipitant solution (like hexadecyltrimethyl ammonium bromide) that makes the intact pectin opaque, thus highlighting the clear zones where pectin has been hydrolyzed.
- Examples:HiMedia Laboratories mentions that certain bacteria like Erwinia carotovora and Erwinia chrysanthemi are known pectin degraders. A document on Scribd indicates that Aspergillus brasiliensis and Fusarium moniliforme are also known to be positive for polygalacturonase production when tested with this medium.
- Applications:MP-5 Medium is useful in various applications, including food microbiology, plant pathology, and general microbial identification.
Pseudomonas Agar For Fluorescein
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₹ 4755 / Litre Get Latest Price
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| Packaging Size | 500ml |
| Packaging Type | Packet |
| Packaging Details | Plastic Bottle |
| Form | Powder |
| Usage/Application | Laboratory/R&D |
| Brand | Himedia |
| Country of Origin | Made in India |
| Product Code | M120 |
| Packing Size | 500G |
| Pack Size | 500 ML |
Minimum order quantity: 1 Litre
Pseudomonas Agar for Fluorescein is a specialized growth medium used to identify and cultivate Pseudomonas species, particularly focusing on their ability to produce fluorescein, a fluorescent pigment. It's a selective medium that enhances fluorescein production while inhibiting the formation of other pigments like pyocyanin. Here's a more detailed description:Purpose: To promote the growth and differentiation of Pseudomonas species based on their ability to produce fluorescein, a yellow-green fluorescent pigment. Composition:
- Peptones: Provide essential nitrogenous compounds, amino acids, and vitamins.
- Magnesium sulfate: Stimulates fluorescein production.
- Dipotassium phosphate: Helps to enhance fluorescein production and suppress pyocyanin.
- Agar: Serves as a solidifying agent.
- Appearance of the powder: Light beige, free-flowing, and homogeneous.
- Appearance of the prepared medium: Light yellow to yellow, slightly opalescent gel.
- The medium is formulated to encourage the production of fluorescein, a water-soluble, chloroform-insoluble pigment produced by some Pseudomonas species.
- This pigment diffuses into the agar surrounding the bacterial colonies, creating a characteristic yellow-green fluorescence when observed under ultraviolet light.
- It is recommended by the U.S. Pharmacopeia for microbial limit tests.
- It helps differentiate Pseudomonas species based on their ability to produce fluorescein.
- It inhibits the production of pyocyanin, another pigment produced by some Pseudomonas species, allowing for clearer observation of fluorescein production.
Antibiotic Assay Medium No 19
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₹ 5515 / Piece Get Latest Price
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| Physical State | Powder |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory/Pharmaceutical or R&D |
| Color | Yellow |
| Brand | Himedia |
| Country of Origin | Made in India |
| Packing Size | 500G |
| Product Code | M101 |
Minimum order quantity: 1 Piece
Antibiotic Assay Medium No. 19 is a microbiological medium used for the bioassay of various antibiotics, particularly Amphotericin B, Nystatin, and Candicidin, using Saccharomyces cerevisiae as the indicator organism. It is a nutrient-rich medium providing essential elements for microbial growth and is used in both the base and seed agar for diffusion assays. Here's a more detailed description:Composition:
- Peptone (9.4 g/L): Provides a source of nitrogen, vitamins, and amino acids.
- Yeast Extract (4.7 g/L): Supplies vitamins, amino acids, and other growth factors.
- Beef Extract (2.4 g/L): Contributes to the nutrient base with nitrogen and other essential components.
- Dextrose (10.0 g/L): Acts as a carbon and energy source for microbial growth.
- Sodium Chloride (10.0 g/L): Maintains the osmotic balance of the medium, crucial for cell integrity.
- Agar (23.5 g/L): A solidifying agent that forms a gel base for the assay.
- Antibiotic Assays:Specifically designed for the microbiological assay of antibiotics like Amphotericin B, Nystatin, and Candicidin, which are antifungal agents.
- Cylinder Plate Method:Commonly used with the cylinder plate method (also known as the cup-plate method) where antibiotic solutions diffuse from cylinders into the agar, creating zones of inhibition that are measured to determine antibiotic potency.
- Seed and Base Agar:Employed as both the base and seed medium in agar diffusion assays.
- USP/EP Compliance:Formulated according to the specifications of the United States Pharmacopeia (USP) and the European Pharmacopoeia (EP).
- Nutrient-rich:Provides a wide range of nutrients for the growth of Saccharomyces cerevisiae.
- Osmotic Balance:Sodium chloride maintains the correct osmotic pressure for cell stability.
- Standardized:The formula is adjusted and standardized to ensure consistent and reliable results in antibiotic assays.
- BSE/TSE Free:In some formulations, such as Antibiotic Veg Assay Medium No. 19, vegetable peptones replace animal peptones, eliminating risks associated with bovine spongiform encephalopathy (BSE) and transmissible spongiform encephalopathy (TSE).
Antibiotic Assay Medium No 36
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₹ 4275 / Piece Get Latest Price
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| Physical State | Powder |
| Packaging Details | Plastic Bottle |
| Packaging Size | 500GM |
| Usage/Application | Laboratory/Microbiological/Pharmaceutical or R&D |
| Color | Yellow |
| Brand | Himedia |
| Country of Origin | Made in India |
| Packing Size | 500G |
| Product Code | M1666 |
Minimum order quantity: 1 Piece
Antibiotic Assay Medium No. 36 is a specialized microbiological medium used for antibiotic assays, particularly for determining the potency of antibiotics. It is a general-purpose medium recommended for cultivating a wide range of microorganisms, including both fastidious and non-fastidious species. This medium is also used for sterility testing in pharmaceutical procedures and is referenced in the US Pharmacopeia. Key Features and Uses:
- Antibiotic Assays:It's a standard medium for antibiotic assays, especially for bleomycin, using Mycobacterium smegmatis as the test organism.
- Microorganism Cultivation:It supports the growth of various microorganisms, making it suitable for a broad range of applications, including isolation and cultivation.
- Sterility Testing:It is used for sterility testing in pharmaceutical settings, ensuring the absence of microbial contamination in products.
- Seed Agar:It is often used as a seed agar for agar diffusion assays, a method for determining antibiotic potency.
- Pharmaceutical Applications:It is widely used in pharmaceutical manufacturing for quality control and potency determination of antibiotics.
- Maintenance of Cultures:It can be used to maintain stock cultures of microorganisms like Pseudomonas aeruginosa for antibiotic testing.
- Freshly Prepared Plates:For accurate antibiotic assays, freshly prepared plates of Antibiotic Assay Medium No. 36 are recommended.
- Sterilization:All materials and media must be properly sterilized before use to prevent contamination.
- Storage:Store the dehydrated powder in a dry place, away from direct sunlight, and tightly sealed to prevent moisture absorption.
Antibiotic Assay Medium No 32
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₹ 5515 / Piece Get Latest Price
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| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | Yellow |
| Country of Origin | Made in India |
| Packing Size | 500G |
| Product Code | M1141 |
Minimum order quantity: 1 Piece
Antibiotic Assay Medium No. 32 is a specialized culture medium used for preparing an inoculum of Bacillus spizizenii to test the potency of dihydrostreptomycin and vancomycin. It is formulated to provide the necessary nutrients and growth factors for the microorganism. This medium is a modified version of Antibiotic Assay Medium No. 1, and is used for plate assays of these antibiotics. Here's a more detailed breakdown:
- Purpose:Used to develop an inoculum of Bacillus spizizenii for testing the potency of dihydrostreptomycin and vancomycin.
- Key Components:Contains essential nutrients, vitamins, minerals, trace elements, and growth factors supplied by peptone, trytone, yeast extract, and HM peptone B.
- Carbon Source:Dextrose is included as a carbon source to stimulate the growth of the test microorganism.
- Growth and Sporulation:Manganese sulfate facilitates sporulation and growth of Bacillus subtilis, a common test organism used in these assays.
- Formulation:The medium is prepared according to the United States Pharmacopoeia and Food and Drug Administration specifications.
Antibiotic Assay Medium No. 36
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₹ 4500 / Kg Get Latest Price
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| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | Yellow |
| Country of Origin | Made in India |
| Packing Size | 500G |
| Produc Code | M1666 |
Minimum order quantity: 1 Kg
Antibiotic Assay Medium No. 36 is a Soya Bean Casein Digest Agar, also known as Tryptone Soya Agar, used primarily for microbiological assays to determine the potency of antibiotics. It serves as a seed agar in antibiotic assays like the agar diffusion assay and is also used for the cultivation of various fastidious and non-fastidious microorganisms, as well as for sterility testing. Key Characteristics:
- Composition:Made from Soya Bean Casein Digest, it provides nutrients to support the growth of a broad range of microorganisms, including fastidious ones.
- Application in Antibiotic Assays:It's used as a seed agar in the cylinder-plate method to measure the zone of inhibition caused by an antibiotic, and also for turbidimetric assays where growth in liquid culture is measured.
- Microbiological Uses:
- Cultivation: It supports the growth of a wide variety of microorganisms, including fastidious organisms like Neisseria and Brucella, and non-fastidious ones.
- Isolation: Used for isolating microorganisms from various specimens.
- Maintenance: Serves as a maintenance medium for stock cultures and specific organisms, such as Mycobacterium smegmatis for bleomycin assays.
- Regulatory Compliance:It is recommended by various pharmacopoeias and conforms to United States Pharmacopoeia (USP) standards for use in pharmaceutical procedures.
- Inhibitor-Free:Its simple, inhibitor-free composition makes it suitable for detecting antimicrobial agents in products and for other microbial limit tests.
Medium 8 MacConkey Agar
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₹ 3835 / Kg Get Latest Price
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| Brand | Himedia |
| Packaging Size | 500 gm |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory/Pharmaceutical or R&D |
| Color | White |
| Country of Origin | Made in India |
| Packing Size | 500G |
| Product Code | MM081 |
Minimum order quantity: 1 Kg
MacConkey Agar Medium 8 is a selective and differential medium used to isolate and differentiate Gram-negative bacteria, particularly enteric pathogens like E. coli and Salmonella. It achieves this by inhibiting Gram-positive bacteria with bile salts and crystal violet, and differentiating lactose-fermenting organisms (which appear pink to red) from non-lactose fermenters (which appear colorless or normal). Key Features:
- Selective:Bile salts and crystal violet inhibit the growth of most Gram-positive bacteria.
- Differential:Differentiates lactose fermenting (pink/red) and non-lactose fermenting (colorless) Gram-negative bacteria.
- Uses:Isolation and identification of enteric bacteria from various sources like clinical specimens, food, and water.
- Principle:Lactose fermentation results in acid production, which lowers the pH and causes the neutral red indicator to turn pink or red in lactose-fermenting bacteria.
- Components:Peptone, lactose, bile salts, crystal violet, neutral red, and agar.
- Bile salts and crystal violet inhibit the growth of most Gram-positive bacteria, allowing Gram-negative bacteria to grow.
- Lactose-fermenting bacteria metabolize lactose, producing acid as a byproduct.
- The acid lowers the pH, causing the neutral red indicator to turn pink or red in the colonies of lactose fermenters.
- Non-lactose fermenting bacteria do not produce acid, so their colonies remain colorless or the original color of the medium.
- Escherichia coli is a lactose fermenter, producing pink to red colonies with bile precipitates.
- Salmonella and Shigella species are non-lactose fermenters, producing colorless or transparent colonies.
Hybridoxl Dmso Sterile Filtered
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₹ 16270 / Piece Get Latest Price
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| Packaging Size | 100 ml |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | colourless |
| Country of Origin | Made in India |
| Shelf Life | 48 months |
| Pack Size | 100ML |
| Product Code | TC433 |
| pH | 7.20 - 7.80 |
| Appearance | Clear colourless liquid |
| Storage | Room Temperature |
| CAS No | 67-68-5 |
| Molecular Formula | C2H6OS |
| Molecular Weight | 78.13 |
Minimum order quantity: 1 Piece
HybridoXL™ DMSO Sterile Filtered is a sterile, filter-sterilized, hybridoma-tested DMSO used in cell culture for cryopreservation and other applications. It's a cryoprotectant used to prevent ice crystal formation during freezing and to protect cells from damage. DMSO is commonly used in cell freezing media at a concentration of 10% (v/v) and can be used for various cell lines and tissues. Here's a more detailed breakdown:
- Function:HybridoXL™ DMSO is a cryoprotectant that prevents ice crystal formation during cell freezing, which can cause damage to cells.
- Use:It's used for cryopreservation of animal cells and tissues, including human embryos and blood cells.
- Concentration:Typically used at a 10% (v/v) concentration in cell freezing media.
- Other Applications:It can also be used to induce cell differentiation and has applications in molecular biology, such as PCR.
- Features:HybridoXL™ DMSO is sterile-filtered and hybridoma-tested, ensuring quality and suitability for hybridoma cell line cryopreservation.
- Safety:It is generally considered safe for cell culture, with most cell lines tolerating up to 1% DMSO without significant toxicity.
Hanks Balanced Salt Solution 1X
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₹ 2410 / Piece Get Latest Price
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| Packaging Size | 500 ml |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | Red |
| Country of Origin | Made in India |
| Shelf life | 24 months |
| Product Code | TL1003 |
| Pack Size | 500 |
| Appearance | Red colored, clear solution |
| pH | 7.20 -7.80 |
| Toxicity test | Passes |
| Storage | 15-30 C |
Minimum order quantity: 1 Piece
Hanks' Balanced Salt Solution (HBSS) 1X is a buffered salt solution used in various cell culture applications. It maintains pH and osmotic balance, provides essential ions, and can be used for rinsing, diluting, or transporting cells and tissues. HBSS is often used in cell culture to maintain a physiological environment, especially for cells maintained in non-CO2 conditions. Key Features and Uses:
- Maintenance of pH and osmotic balance:HBSS helps maintain the proper pH and osmotic pressure within cells, which are crucial for their survival and function.
- Provision of essential ions:It provides cells with the necessary inorganic ions, like sodium, potassium, calcium, and magnesium, that are essential for various cellular processes.
- Washing, diluting, and transporting cells:HBSS is used to rinse cells before dissociation, dilute cells for counting, and transport cells or tissue samples.
- Diluent and reagent preparation:It serves as a diluent for various cell culture reagents and can be used to prepare buffers.
- Phenol Red:Some HBSS formulations contain phenol red, an indicator that changes color with pH changes. Others are phenol red-free.
- Calcium and Magnesium:Some HBSS formulations include calcium and magnesium, while others are calcium and magnesium-free.
- CO2 dependency:HBSS is designed to equilibrate with air and doesn't require a CO2 environment, making it suitable for cells maintained in non-CO2 conditions.
Sucrose Plant Culture Tested
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₹ 4450 / Kg Get Latest Price
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| Packaging Size | 5kg |
| Brand | Himedia |
| Packaging Details | Plastic Can |
| Usage/Application | Laboratory |
| Color | Colourless |
| Country of Origin | Made in India |
| Shelf life | 4 years |
| Product Code | PCT0607 |
| Pack Size | 5KG |
| CAS No | 57-50-1 |
| Molecular Formula | C12H22O11 |
| Molecular Weight | 342.30 |
| HS Code | 17019990 |
| Storage | -30C |
| Appearance | Colourless or white hygroscopic crystals or powder or solid |
| Form | Solid |
Minimum order quantity: 1 Kg
Sucrose, also known as table sugar, is a disaccharide made of glucose and fructose. It is widely used in plant culture as a primary energy source, providing carbon and energy for plant cells. Sucrose, particularly for plant tissue culture, is available in different grades (e.g., Grade I, Grade II) and is typically tested for purity and quality. Here's a more detailed look:
- Chemical Properties:Sucrose has the chemical formula C12H22O11 and a molecular weight of 342.30.
- In Plant Culture:It serves as a major carbohydrate source, providing energy to plant cells during in vitro culture.
- Purity:Sucrose used in plant culture is often tested for impurities like heavy metals and sulfate.
- Grades:Different grades of sucrose (e.g., Grade I, Grade II) are available, with Grade I typically being of higher purity.
- Specific Applications:Sucrose can be used in various plant culture applications, including micropropagation, callus induction, and as a cryoprotectant.
- Effects of Concentration:Sucrose concentration in the culture medium can influence plant growth, with optimal concentrations being crucial for various processes like rhizogenesis and callogenesis.
HAT Medium Supplement 50X Liquid
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₹ 8590 / Piece Get Latest Price
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| Packaging Size | 5X10ML |
| Brand | Himedia |
| Packaging Details | Glass Bottle |
| Usage/Application | Laboratory |
| Color | Colorless |
| Country of Origin | Made in India |
| Physical state | Liquid |
| Product Code | TCL072 |
| Pack Size | 5x10ML |
| Odour | Characteristic |
| pH | 8.90 - 9.50 |
| Storage | -30 C to -10 C |
Minimum order quantity: 1 Piece
TCL072 is a sterile filtered 50X HAT medium supplement containing 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline. HAT supplement contains Hypoxanthine, Aminopterin and Thymidine and is used for the preparation of selection medium for hybridoma
TCL072 from HiMedia is a sterile, liquid, 50X concentration supplement for HAT medium (Hypoxanthine-Aminopterin-Thymidine), crucial for hybridoma selection in antibody production, providing essential components (hypoxanthine, aminopterin, thymidine in PBS) to select for fused cells that can survive while killing unfused cells and other unwanted cells in cell culture. Key Details:
TCL072 from HiMedia is a sterile, liquid, 50X concentration supplement for HAT medium (Hypoxanthine-Aminopterin-Thymidine), crucial for hybridoma selection in antibody production, providing essential components (hypoxanthine, aminopterin, thymidine in PBS) to select for fused cells that can survive while killing unfused cells and other unwanted cells in cell culture. Key Details:
- Product Name: HAT Medium Supplement 50X, Liquid.
- Catalog No.: TCL072.
- Components: Contains Hypoxanthine (680.5 mg/L), Aminopterin (8.8 mg/L), and Thymidine (193.8 mg/L) in Phosphate Buffered Saline (PBS).
- Concentration: 50X (used to prepare 1X working medium).
- Function: A selection agent in hybridoma technology, allowing only cells with intact salvage pathways (like B-cell/myeloma hybrids) to survive and proliferate, while killing HGPRT-deficient cells.
- Application: Used in generating monoclonal antibodies by selecting hybridoma cells for indefinite culturing and antibody production. The HiMedia TCL072 is a 50X concentrated, sterile-filtered liquid supplement for HAT (Hypoxanthine, Aminopterin, Thymidine) selection medium, crucial for hybridoma cell culture, containing specific concentrations of Hypoxanthine (680.5 mg/L), Aminopterin (8.8 mg/L), and Thymidine (193.8 mg/L) in Phosphate Buffered Saline (PBS), used to select for fused B-cell/myeloma hybrid cells in research. Key Specifications & Components:
- Product Code: TCL072 (also TCL072-10X10ML).
- Type: HAT Medium Supplement, 50X concentration, Liquid.
- Components:
- Hypoxanthine: 680.5 mg/L.
- Aminopterin: 8.8 mg/L.
- Thymidine: 193.8 mg/L.
- Solvent: Phosphate Buffered Saline (PBS).
- Filtration: Sterile filtered.
- Application: Cell culture for hybridoma production (Monoclonal Antibody production).
- Aminopterin's Role: Blocks the de novo pathway of nucleotide synthesis.
- Cellular Deficiency: Myeloma cells lack HGPRT (Hypoxanthine-Guanine Phosphoribosyltransferase) or TK (Thymidine Kinase) and can't use salvage pathways.
- Selection:
- Unfused B-cells die off quickly.
- Myeloma cells die because they can't make DNA.
- Only hybrid cells (containing both B-cell & myeloma genes) survive, as they get the necessary nucleotides from the salvage pathway.
- It's added to cell culture media (like DMEM) to create the selective HAT environment.
- After selection, cells are often moved to HT (Hypoxanthine-Thymidine) medium to dilute out aminopterin before final culturing.
DNase I Solution 1mg
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₹ 1525 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 1ML |
| Brand | HIMEDIA |
| Packaging Details | Box |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Product Code | ML068 |
Minimum order quantity: 1 Bottle
DNase I is a deoxyribonuclease enzyme that specifically cleaves phosphodiester bonds in DNA, leading to the degradation of both single- and double-stranded DNA into shorter oligonucleotide fragments. Its activity is heavily influenced by the presence of divalent metal ions, particularly Mg²⁺ and Ca²⁺, which are essential for optimal enzymatic function.Key Features and Properties
For detailed handling instructions and specific protocols, refer to the product datasheets from the respective manufacturers.
- Source and Form: DNase I is typically prepared from bovine pancreas and is available either as a lyophilized powder or in solution form (e.g., 1 mg/mL) for immediate use in lab procedures.
- Enzymatic Activity: The enzyme has a minimum activity of 3000 U/mg (Kunitz) and releases 5’-phosphorylated and 3’-hydroxylated ends upon digestion 3.
- Storage conditions: Generally, it is recommended to store DNase I at -20°C, avoiding frost-free freezers due to temperature fluctuations. Once reconstituted, it should be kept on ice until used and can be aliquoted and frozen for stability 2.
- Heat Inactivation: DNase I can be inactivated by heating to 75°C for 10 minutes or by incorporating chelators like EDTA2 .
- 5 Sources
- Removing genomic DNA from RNA preparations prior to reverse transcription or PCR, ensuring downstream applications are DNA-free 1.
- Avoiding cell clumping during tissue disaggregation: the enzyme degrades released DNA from ruptured cells, preventing aggregation in concentrated or cryopreserved cell suspensions 2.
- Nick Translation and Footprinting Studies: DNase I is utilized in generating marked DNA for labeling purposes and in footprinting assays to study DNA-protein interactions 1.
- 4 Sources
- Always use RNase-free and DNase-free materials when working with DNase I to prevent contamination and ensure the integrity of nucleic acids 1.
- Avoid vigorous mixing or vortexing of DNase I solutions as it is sensitive to physical denaturation 2.
- 3 Sources
For detailed handling instructions and specific protocols, refer to the product datasheets from the respective manufacturers.
Potato Dextrose Agar
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₹ 3990 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory/Pharmaceutical or R&D |
| Color | White |
| Brand | Himedia |
| Country of Origin | Made in India |
| Product Code | MH096 |
| Pack Size | 500ML |
Minimum order quantity: 1 Kg
Potato Dextrose Agar (PDA) is a microbiological growth medium widely used for cultivating fungi, particularly yeasts and molds. It's a nutrient-rich medium composed of potato infusion, dextrose (glucose), and agar, which serves as a solidifying agent. PDA is favored for its ability to support the growth of various fungi, including those found in food, dairy products, and even clinical samples. Key Components and Purpose:
- Potato Infusion: Provides a source of nutrients and growth factors for fungi.
- Dextrose (Glucose): Acts as a readily available carbohydrate source for energy.
- Agar: A solidifying agent that creates a stable surface for microbial growth.
- Cultivating Fungi:PDA is a standard medium for growing and isolating fungi, including yeasts and molds.
- Enumeration of Fungi:Used to count the number of fungal colonies in a sample.
- Studying Fungal Morphology:The medium allows for observation of colony characteristics and pigment production in some fungi.
- Fungal Identification:Certain fungal species can be identified based on their growth patterns and colony characteristics on PDA.
- Testing for Fungal Contamination:PDA is used in food and dairy industries to assess the presence and level of fungal contamination.
- Antimicrobial Agent Testing:Can be used to evaluate the effectiveness of antimicrobial agents against fungi.
- Acidification:PDA can be acidified (e.g., with tartaric acid) to inhibit bacterial growth, making it more suitable for fungal isolation.
- Supplementation with Antibiotics:Antibiotics like chloramphenicol can be added to suppress bacterial growth, allowing for selective fungal growth.
- Copper Supplementation:Some studies have shown that adding copper to PDA can enhance pigmentation in certain fungal cultures, according to a study published in FEMS Microbiology Letters.
Peptone Water Himedia
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₹ 3705 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | HIMEDIA |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | White |
| Country of Origin | Made in India |
| Packaging type | HDPE |
| Physical Form | Powder |
| Pack Size | 500ML |
| HSN CODE | 38220000 |
Minimum order quantity: 1 Bottle
Peptone Water, particularly from HiMedia, is a widely used microbiological medium. It serves as a general-purpose growth medium for non-fastidious microorganisms and also acts as a base for carbohydrate fermentation studies. It's composed of peptic digest of animal tissue and sodium chloride, providing essential nutrients like nitrogen and maintaining osmotic balance. Here's a more detailed breakdown:Key Components and Properties:
- Peptone: Provides nitrogenous and carbonaceous compounds, amino acids, and vitamins, supporting bacterial growth.
- Sodium Chloride: Maintains the osmotic balance of the medium, crucial for cell viability.
- pH: Typically adjusted to 7.2 ± 0.2 at 25°C, but can be adjusted for specific applications like Vibrio cultivation (pH 8.4).
- Appearance: Usually a light yellow to yellowish-brown powder.
- Solubility: Readily soluble in water.
- General Growth Medium:Supports the growth of a wide variety of microorganisms, especially those not requiring highly specific growth factors.
- Carbohydrate Fermentation Studies:A basal medium for studying the ability of organisms to ferment specific carbohydrates. By adding a carbohydrate and a pH indicator (like phenol red), researchers can observe acid production (indicated by a color change) and gas production (using Durham tubes).
- Indole Production Testing:Specifically recommended for detecting indole production by Escherichia coli.
- Enrichment of Vibrio Species:Peptone water with a pH of 8.4 is particularly useful for cultivating Vibrio species.
- Peptone Water (general): The basic formulation.
- Peptone Water with Phenol Red: Contains phenol red indicator for carbohydrate fermentation studies.
- Peptone Water with pH adjusted to 8.4: Specifically for Vibrio species.
- Andrade Peptone Water: A basal medium for carbohydrate fermentation with Andrade's indicator.
- Buffered Peptone Water: Used for pre-enriching damaged Salmonella species from food samples.
Collagenase Type IV Solution
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₹ 12745 / Piece Get Latest Price
Product Brochure
| Packaging Size | 100 ml |
| Grade | LR (Laboratory) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | Orange |
| Country of Origin | Made in India |
| Product Code | TCL117 |
| Pack Size | 100Ml |
| Make | Himedia |
| Appearance | Orange colored, clear solution |
| Type | Solution |
| Hersteller | NeoFroxx |
| Applikationen | IV |
Minimum order quantity: 1 Piece
Collagenase Type IV is a specialized enzyme that breaks down type IV collagen, a key component of the basement membrane. It's derived from bacteria like Clostridium histolyticum. This enzyme is used in various applications, including isolating islet cells, dissociating tissues, and degrading extracellular matrix. Key Features and Applications:
- Specificity:Collagenase Type IV has a specific affinity for the sequence R-Pro-(X-Gly-Pro), where X is often a neutral amino acid, allowing it to target type IV collagen.
- Tissue Dissociation:It's effective in breaking down the intercellular matrix, which is crucial for separating cells from tissues.
- Basement Membrane Degradation:It can degrade both type IV and type VII collagen, which are major components of the basement membrane.
- Applications:
- Islet Isolation: Recommended for isolating Islets of Langerhans, which are important for insulin production.
- Cell Dissociation: Used in cell culture for isolating cells from tissues, especially when dealing with dense connective tissues.
- Stem Cell Research: Used in various stem cell research areas, including reprogramming, maintenance, and differentiation of both human and mouse pluripotent stem cells.
- Storage and Preparation:
- Powdered Form: Stored at 2-8°C.
- Solution: Reconstituted solution should be stored at -20°C.
- Stock Solution: A 100mg/mL stock solution can be prepared by dissolving the powder in Hank's Balanced Salt Solution (HBSS).
- Filtering: The stock solution should be filter-sterilized before use.
- Working Concentration:The optimal working concentration needs to be determined based on specific experimental conditions and the type of tissue being dissociated.
- Secondary Proteases:Some collagenase preparations may contain clostripain and other secondary proteases, which can be a concern if they damage membrane proteins or receptors. Collagenase Type IV is designed to minimize these activities.
- Safety:Collagenase is used in research and may not be suitable for direct human application without proper evaluation and approval.
Soyabean Casein Digest Medium Himedia
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₹ 2125 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | White |
| Country of Origin | Made in India |
Minimum order quantity: 1 Kg
Casein Soyabean Digest Broth, also known as Soybean Casein Digest Medium (SCDM) or Tryptone Soya Broth (TSB), is a widely used general-purpose microbiological medium for cultivating a broad range of bacteria and fungi. It's a nutrient-rich broth that supports the growth of both fastidious and non-fastidious microorganisms. Key Characteristics and Components:
- General-Purpose Medium: SCDM is versatile and suitable for various applications, including sterility testing, microbial limit testing, and general cultivation in research and quality control.
- Nutrient Rich: It contains papaic digest of soybean meal and enzymatic digest of casein, providing a rich source of amino acids, peptides, and other nutrients for microbial growth.
- Composition: Common ingredients include soybean meal digest, casein digest, dipotassium phosphate, sodium chloride, and glucose.
- Appearance: Typically a light beige, free-flowing powder that dissolves in water.
- pH: The typical pH of the prepared medium is around 7.3 ± 0.2.
- Applications: SCDM is used for:
- Cultivating a wide variety of bacteria and fungi.
- Sterility testing of pharmaceuticals, food products, and other materials.
- Microbial limit testing to determine the total viable count of microorganisms.
- General microbiological research and quality control.
- Alternative Formats: SCDM is also available in agar form (Soybean Casein Digest Agar or Tryptone Soya Agar) for plate-based cultivation.
Bhi Broth Brain Heart Infusion Broth Himedia
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₹ 1015 / Kg Get Latest Price
Product Brochure
| Packaging Size | 100 g |
| Grade | AR (Analytical) |
| Brand | HIMEDIA |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Packaging type | HDPE |
| Pack Size | 100g |
| Physical Form | Powder |
| Recommended Storage Temperature | On receipt store between 10-30°C |
| Specific end uses | Apart from the uses mentioned in section 1.2 no other specific uses are stipulated |
| Appearance | Cream to yellow coloured homogeneous free flowing powder |
| pH | 7.20 - 7.60 |
| Form / Physical state | Dehydrated powder, cream to yellow, homogeneous, free‑flowing |
| Standards / Performance | The composition and performance criteria conform to ISO 6880:1983 (as per Hi |
Minimum order quantity: 1 Kg
Brain Heart Infusion (BHI) Broth is a highly nutritious, buffered liquid culture medium used to grow a wide variety of microorganisms, including fastidious and pathogenic bacteria, fungi, and yeasts. It is made from an infusion of animal heart and brain, providing amino acids, carbon, vitamins, and minerals, along with glucose and a buffering agent like disodium phosphate. BHI Broth is versatile and can be enriched with other components like blood, hemin, or vitamin K to support the growth of specific organisms for diagnostic and research purposes. Key features
- Nutrient-rich: Contains the necessary components for a broad range of microbial growth, including amino acids, vitamins, and carbohydrates from brain-heart infusion and glucose.
- Buffered: The addition of disodium phosphate helps maintain a stable pH, which is crucial for microbial survival and growth.
- Versatile: Can be modified with additives for specific applications.
- Cultivating microorganisms: Used for the general cultivation of bacteria, including pathogenic cocci and fastidious organisms.
- Fungal and yeast growth: Suitable for culturing yeasts and molds; with the addition of sheep blood, it can be used for isolating fungi like Histoplasma capsulatum.
- Blood cultures: Used as a medium for setting up blood cultures.
- Antimicrobial susceptibility testing: Used to prepare bacterial inocula for susceptibility testing.
- Diagnostic tests: Specifically suited for cultivating Staphylococcus for the plasma coagulase test.
- For anaerobic growth: Enriched with vitamin K and hemin.
- For Haemophilus species: Enriched with Fildes enrichment, hemin, and NAD.
- For Gonococci: Can be supplemented with ascites.
Baird Parker Agar Base w/ Sulpha Himedia
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₹ 7100 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Packaging type | HDPE |
| Pack Size | 500g |
| Recommended Storage Temperature | On receipt store between 10-30°C |
| Specific end uses | Apart from the uses mentioned in section 1.2 no other specific uses are stipulated. |
| Appearance | Cream to yellow coloured homogeneous free Page 4 of 8 flowing powder |
| pH | 6.80 - 7.20 |
| Tryptone | 10.000 |
| HM Peptone B # (equivalent to beef extract) | 5.000 |
| Yeast Extract | 1.000 |
| Glycine | 12.000 |
| Sodium Pyruvate | 10.000 |
| Lithium Chloride | 5.000 |
| Sulphamethazine (Sulpha) | 0.050 |
| Agar | 20.000 |
| Final pH (at 25 °C) | 7.0 ± 0.2 |
| Physical Form | Powder |
Minimum order quantity: 1 Kg
-
- Selective agent: Contains tellurite and lithium chloride to suppress the growth of most bacteria while allowing Staphylococci to grow.
- Growth promoters: Includes pyruvate and glycine to promote the growth of Staphylococci.
Baird Parker Agar Base w/ Sulpha is a selective culture medium used for the isolation and enumeration of coagulase-positive Staphylococcus species, particularly Staphylococcus aureus, from food and other samples. It contains components that inhibit the growth of other microorganisms while promoting the growth of Staphylococci, and it allows for a clear differentiation of Staphylococci colonies based on their metabolic reactions, which include the reduction of tellurite and the breakdown of egg yolk. Key features and components
-
- Differentiation:
- Tellurite reduction: Staphylococci reduce tellurite, causing them to appear as grey, black, or brown-grey colonies.
- Egg yolk breakdown: Colonies of Staphylococcus aureus are surrounded by an opaque zone due to the breakdown of the egg yolk emulsion, which is often accompanied by a clear halo.
- Recommended use:
- Used for the isolation and enumeration of coagulase-positive Staphylococci.
- Recommended by organizations like AOAC International and ISO for use in food and microbial limit testing.
- How to use
- Suspend the specified amount of agar base in distilled or de-ionized water.
- Heat to dissolve the powder completely.
- Autoclave the medium at 121∘C121 raised to the composed with power cap C121∘𝐶 for 15 minutes.
- Dispense into sterile containers and allow to cool before adding any required supplements, such as the egg yolk emulsion.
- Inoculate the plates with the sample and incubate at the specified temperature.
- Differentiation:
Lactobacillus MRS Broth, Granulated (MRS Broth, Granulated) Himedia
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₹ 5530 / Bottle Get Latest Price
Product Brochure
| Media Type | Dehydrated Culture Media (Broth). |
| Packaging Size | 500 g |
| Brand | HiMedia |
| Usage / Application | Analytical / QC, Research, Microbiology, Synthesis |
| Color | cream to yellow |
| Country of Origin | Made in India |
| Pack Size | 500g |
| Packaging type | HDPE |
| Physical Form | Granular |
| Appearance | Cream to yellow granular medium |
| Prepared Medium | Medium amber, clear to slightly opalescent solution |
| Recommended Storage Temperature | On receipt store between 2-8°C |
| Specific end uses | Apart from the uses mentioned in section 1.2 no other specific uses are stipulated |
| pH | 6.30 - 6.70 |
| Bulk density | 620kg/m³ for the granular form (as per Sigma/Millipore product) |
| Sterilization | Autoclave at 121°C for 15 minutes (15psi) |
Minimum order quantity: 1 Bottle
Himedia's Lactobacillus MRS Broth, Granulated is a dehydrated, granular culture medium for the isolation and cultivation of all Lactobacillus species. It is a cream to yellow granular medium that, when prepared, forms an amber-colored, clear to slightly opalescent solution. The medium's composition, which includes sodium acetate and ammonium citrate, selectively inhibits the growth of other microorganisms like streptococci and molds. Product features and specifications
- Product Name: Lactobacillus MRS Broth, Granulated (MRS Broth, Granulated)
- Brand: HiMedia
- Catalog Number: GM369
- Physical Form: Granular
Potassium Hydroxide Flakes Himedia
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₹ 415 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage / Application | Microbiology, Industrial Use, Research, Analytical / QC, Synthesis |
| Usage/Application | Laboratory |
| Color | White to slightly yellow hygroscopic flakes |
| Country of Origin | Made in India |
| Physical state | solid |
| Shelf Life | 4 years |
| Pack Size | 500g |
| CAS No | 1310-58-3 |
| Molecular formula | KOH |
| Recommended storage temperature | 10–30 °C |
| Odour | characteristic |
| Solubility | Highly soluble in water |
| Synonyms | Caustic potash, lye, potash lye |
| Appearance | White to slightly yellow flakes |
Minimum order quantity: 1 Kg
Himedia's Potassium Hydroxide Flakes (KOH) is an inorganic, white to slightly yellow, hygroscopic compound, commonly known as caustic potash. It is used as a raw material for synthesizing other potassium compounds, as an activator in chemical processes like activated carbon preparation, and as a base for N-alkylation reactions. This product is for laboratory, diagnostic, research, or further manufacturing use and is not intended for human or animal consumption.
Nutrient HiVeg Agar Himedia
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₹ 3515 / Gram Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Brand | HIMEDIA |
| Prescription/Non prescription | Prescription |
| Product Code | MV001-500G |
| Form | Powder |
| Shelf Life | 36 Months |
Minimum order quantity: 100 Gram
The HiMedia product with catalog number MV001 is Nutrient HiVeg™ Agar, a dehydrated culture medium used in microbiology. Description and Intended Use
- Intended Use: It is a general-purpose, non-selective medium used for the cultivation and enumeration of less fastidious microorganisms. It can be enriched with blood or other biological fluids to grow more fastidious organisms.
- Principle: The medium provides essential nitrogen compounds, carbon, vitamins, and trace ingredients necessary for bacterial growth, with sodium chloride maintaining osmotic equilibrium.
- HiVeg™ Advantage: This formulation uses plant-based peptones (HiVeg) instead of animal-based ones, making it a vegetarian product.
- Appearance: Cream to yellow, homogeneous free-flowing powder.
- pH: The reaction of a 2.8% w/v aqueous solution at 25°C is pH 7.4 ± 0.2.
- Gelling: Forms a firm gel comparable to a 1.5% agar gel.
- Color and Clarity: Light yellow, clear to slightly opalescent gel forms in Petri plates.
- Suspend 28.0 grams of the powder in 1000 ml of purified/distilled water.
- Heat to boiling to fully dissolve the medium.
- Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
- Cool to 45-50°C. If enrichment is desired, add 5-10% blood or other sterile biological fluids.
- Mix well and pour into sterile Petri plates.
- Intended Use: It is a general-purpose, non-selective medium used for the cultivation and enumeration of less fastidious microorganisms. It can be enriched with blood or other biological fluids to grow more fastidious organisms.
- Principle: The medium provides essential nitrogen compounds, carbon, vitamins, and trace ingredients necessary for bacterial growth, with sodium chloride maintaining osmotic equilibrium.
- HiVeg™ Advantage: This formulation uses plant-based peptones (HiVeg) instead of animal-based ones, making it a vegetarian product.
- Appearance: Cream to yellow, homogeneous free-flowing powder.
- pH: The reaction of a 2.8% w/v aqueous solution at 25°C is pH 7.4 ± 0.2.
- Gelling: Forms a firm gel comparable to a 1.5% agar gel.
- Color and Clarity: Light yellow, clear to slightly opalescent gel forms in Petri plates.
- Suspend 28.0 grams of the powder in 1000 ml of purified/distilled water.
- Heat to boiling to fully dissolve the medium.
- Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
- Cool to 45-50°C. If enrichment is desired, add 5-10% blood or other sterile biological fluids.
- Mix well and pour into sterile Petri plates.
MacConkey Agar w/ CV and w/o NaCl
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₹ 4010 / Gram Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | White |
| Country of Origin | Made in India |
| Product Code | M1582 |
| Pack Size | 500ML |
Minimum order quantity: 1 Gram
MacConkey agar with crystal violet (CV) and without sodium chloride (NaCl) is a differential and selective medium used to isolate and differentiate Gram-negative bacteria, particularly enteric pathogens, while inhibiting the growth of Gram-positive bacteria. It's a modification of the original MacConkey agar formulation, where the absence of NaCl and the presence of CV and bile salts restrict the growth of swarming Proteus species and many Gram-positive organisms. The medium differentiates lactose fermenters (e.g., E. coli) from non-lactose fermenters (e.g., Salmonella, Shigella) by observing colony color. Components and Function:
- Bile Salts and Crystal Violet:These components inhibit the growth of Gram-positive bacteria, making it selective for Gram-negative bacteria.
- NaCl (Absent):The lack of NaCl helps to further restrict the swarming of Proteus species.
- Lactose:Lactose is a fermentable sugar that allows differentiation between lactose-fermenting and non-lactose-fermenting bacteria.
- Neutral Red:This pH indicator turns red in the presence of acid (produced by lactose fermentation), indicating lactose-fermenting colonies.
- 1. Selective Action:Bile salts and crystal violet inhibit the growth of Gram-positive bacteria, while sodium chloride further restricts swarming Proteus.
- 2. Differential Action:Lactose is fermented by some bacteria, producing acid. This acid causes the neutral red indicator to turn red, resulting in pink or red colonies.
- 3. Lactose Non-fermenters:Bacteria that don't ferment lactose will not produce acid, and their colonies will remain colorless or transparent.
- Selective: Inhibits Gram-positive bacteria, allowing for the isolation of Gram-negative bacteria.
- Differential: Distinguishes lactose-fermenting bacteria from non-lactose fermenters.
- Reduces Swarming: The absence of NaCl helps to reduce or prevent swarming of Proteus species.
Tryptone Bile Agar
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₹ 5420 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Brand | Himedia |
| Packaging Details | Glass Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Appearance | Cream to yellow coloured homogeneous free flowing powder |
| pH | 7.00 - 7.40 |
| Pack Size | 500G |
Minimum order quantity: 1 Kg
Tryptone Bile Agar (TBA) is a selective culture medium used for the detection and enumeration of Escherichia coli (E. coli) in food, water, and other samples. It is particularly useful for identifying E. coli strains that may be missed by other methods due to their ability to ferment lactose late or not at all. Here's a more detailed description:Composition:
- Tryptone (pancreatic digest of casein): Provides essential nutrients for bacterial growth.
- Bile salts: Act as a selective agent, inhibiting the growth of Gram-positive bacteria.
- Agar: A solidifying agent that forms a gel, allowing for the formation of colonies.
- Distilled or deionized water: Used as a solvent to dissolve the other ingredients.
- Some formulations may also include other components like sodium chloride or dextrose.
- TBA is formulated to be selective for Gram-negative bacteria, particularly E. coli, by inhibiting the growth of other bacteria.
- It is also designed to be differential, allowing for the identification of E. coli based on its ability to ferment lactose and produce gas.
- For example, E. coli colonies may appear pinkish-red with a bile precipitate when grown on Violet Red Bile Agar (VRBA), a related medium.
- TBA is used in the food industry to test for E. coli contamination in raw meats, and other food products.
- It is also used in water quality testing and clinical microbiology.
- The medium is particularly useful for detecting E. coli strains that are not easily identified by other methods, such as those that ferment lactose late or not at all.
- The modified direct plating method (DPM) is often used with TBA to enumerate E. coli.
- TBA can also be used with the filter membrane method or other direct inoculation methods.
- Tryptone Bile Glucuronic Agar (TBX): A variation of TBA that includes a chromogenic substrate (5-bromo-4-chloro-3-indolyl-β-D-glucuronide) to detect the presence of the enzyme β-glucuronidase, which is produced by most E. coli strains. This allows for easy differentiation of E. coli colonies, which appear blue-green on TBX agar, according to HiMedia.
Sodium Chloride Hi LR Himedia
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₹ 205 / Kg Get Latest Price
Product Brochure
| Purity | ≥98% |
| Form | Powder |
| Brand | Himedia |
| Packaging Size | 500 g |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | Colourless to white crystals or powder or solid |
| Country of Origin | Made in India |
| Physical state | solid |
| CAS No | 7647-14-5 |
| Pack Size | 500g |
| Molecular formula | NaCl |
| Recommended storage temperature | 10–30 °C |
| Odour | characteristic |
| Appearance | solid ionic salt |
| Purity (assay) | Likely very high (e.g. ≥ 99%) |
Minimum order quantity: 1 Kg
Sodium chloride, Hi-LR™ from HiMedia is a laboratory-grade sodium chloride (NaClcap N a cap C l𝑁𝑎𝐶𝑙) that is suitable for many laboratory applications, including use in the development of diagnostic assays. It is a white crystalline powder with a molecular formula of NaClcap N a cap C l𝑁𝑎𝐶𝑙 and a molecular weight of 58.4458.4458.44. The "Hi-LR" designation indicates it meets a high standard of purity, meaning it is suitable for use in laboratories that require reliable and consistent reagents.
S Selective Supplement Himedia
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₹ 1740 / Piece Get Latest Price
Product Brochure
| Packaging Size | 5VL |
| Brand | Himedia |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Pack Size | 5VL |
| Recommended Storage Temperature | On receipt store between 2-8°C |
| Appearance | White powder |
| Potassium | tellurite Inhibits most Gram-negative and some Gram-positive bacteria; allows S. aureus to |
| Lithium | chloride Increases selectivity by inhibiting non-target microbes. |
| Sulphamethazine (Sulpha) | Suppresses swarming Proteus spp. and other competing flora |
| Optional antibiotics (like polymyxin or novobiocin in some variants) | Further enhances selectivity fo |
| Addition to Medium | Aseptically add the contents of one vial to 500mL of appropriate sterile, molte |
Minimum order quantity: 1 Piece
"S Selective Supplement" from Himedia refers to a line of antibacterial supplements, most commonly the S Selective Supplement (FD068) for the selective isolation of Salmonella species. It is a powdered supplement used with a growth medium, such as a base agar, to inhibit the growth of unwanted microorganisms while allowing Salmonella to flourish. These supplements are intended for in vitro diagnostic use by professionals in laboratory settings, particularly for food and clinical sample analysis. Key features of Himedia S Selective Supplement
- Purpose: To selectively isolate Salmonella species from a mixed population of bacteria in laboratory cultures.
- Composition: A sterile, dehydrated powder, typically containing antibiotics and other selective agents.
- Usage: Aseptically rehydrated and added to a sterile, molten growth medium, like a base agar, to create a selective culture plate.
- Application: Used for both clinical samples (like pus and wounds) and food samples, following established laboratory guidelines for handling and sterilization of used materials.
- Target Organism: Salmonella species.
- Other "S" Selective Supplements: Himedia also produces other "S" selective supplements, such as BP S Selective Supplement for suppressing Proteus species and S.F.P. Selective Supplement for Clostridium perfringens.
Tryptophan Medium Himedia
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₹ 3380 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Recommended Storage Temperature | On receipt store between 10-30 DegreeC |
| Pack Size | 500g |
| Appearance | Cream to yellow coloured homogeneous free flowing powder |
| pH | 7.30 - 7.70 |
Minimum order quantity: 1 Kg
HiMedia's Tryptophan Medium is a microbiological culture medium used for the detection of indole production by bacteria. It contains the amino acid tryptophan, which certain bacteria, like Escherichia coli and Vibrio parahaemolyticus, break down into indole. The presence of indole is then detected by adding Kovacs' reagent, which produces a red ring when positive. Different versions of Tryptophan Medium are available, including Tryptophan HiVeg™ Medium (using vegetable peptones) and Saline Tryptone / Tryptophan Medium, which adheres to specific ISO standards. Key Features and Uses
- Indole Production Test:The primary purpose is to test for the ability of microorganisms to produce indole from the amino acid tryptophan.
- Bacterial Differentiation:It aids in the differentiation of bacteria, such as identifying E. coli O157:H7, a key step in coliform differentiation.
- Kovacs' Reagent:Indole production is identified by adding Kovacs' reagent to the culture, resulting in a red ring upon a positive reaction.
- Variety of Formulations:
- Tryptophan HiVeg™ Medium: A version that replaces animal peptones with vegetable-defined peptones to eliminate risks from BSE/TSE/GMOs.
- Saline Tryptone / Tryptophan Medium: This medium follows ISO 8914:1990 standards and is used for detecting indole production by Vibrio parahaemolyticus.
- DEV Tryptophan Broth: Specifically recommended for the subcultivation of coliforms and indole testing in water bacteriology, following German regulations.
- Bacteria capable of indole production are incubated in the medium.
- These bacteria enzymatically break down tryptophan into indole and other products.
- Kovacs' reagent is added, which reacts with the indole to form a red-colored ring at the surface of the broth.
- The formation of the red ring indicates a positive test for indole production.
U40 Supplement Himedia
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₹ 550 / Piece Get Latest Price
Product Brochure
| Packaging Size | 5-VL |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Glass Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Appearance | Colorless clear liquid |
| Product Code | FD048 |
| Urea | 2g |
| Distilled water | 5ml |
| Final pH ( at 25 DegreeC) | 8.0 0.2 |
Minimum order quantity: 1 Piece
U40 supplement from HiMedia is a filter-sterilized urea solution (5 ml per vial) used to detect urease activity in microbiological media. It's specifically recommended for use with Urea Agar Base (Christensen) and is a key component in identifying urease-positive organisms, particularly Proteus vulgaris. The supplement is a slight modification of the Christensen formulation and is recognized by the Bureau of Indian Standards (BIS). Here's a more detailed breakdown:
- Function:U40 supplement is used to detect urease activity, which is the ability of an organism to produce the enzyme urease, which breaks down urea.
- Composition:Each vial contains a urea solution, sufficient for 100 ml of medium.
- Application:It's commonly used with Urea Agar Base (Christensen) for identifying bacteria, especially Proteus vulgaris, which is a rapid urease producer.
- Benefits:The supplement helps differentiate between various urease-positive organisms, including those from the Enterobacteriaceae family, and those that are not.
- Standards:The supplement's use is also recommended by the Bureau of Indian Standards under the specifications IS:5887(Part I)-1976.
- References:Several publications, including those by Christensen, MacFaddin, and the Bureau of Indian Standards, are referenced in its application.
Buffered Peptone Water Himedia
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₹ 3545 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Pack Size | 500g |
| CAS No | 633-03-4 |
| Recommended Storage Temperature : On receipt store between 10-30 DegreeC | On receipt store between 10-30 DegreeC |
| Appearance | Cream to greenish yellow homogenous free flowing powder |
| pH | 7.80 - 8.20 |
Minimum order quantity: 1 Bottle
Himedia's Buffered Peptone Water (BPW) is a non-selective, nutrient-rich pre-enrichment broth designed to recover and resuscitate sub-lethally injured Enterobacteriaceae and other bacteria from food and clinical samples by providing a buffered, inhibitor-free environment. Its composition of proteose peptone, sodium chloride, and a phosphate buffer system supplies nutrients, maintains osmotic balance, and prevents pH changes that could harm stressed microorganisms. It's a recommended medium by international standards like ISO 6579-1:2017 for its role in detecting bacteria such as Salmonella and Cronobacter species prior to selective enrichment. Key Features & Benefits
- Resuscitation of Injured Cells:BPW is formulated to enhance the recovery of bacteria that have been damaged by food processing or other environmental factors.
- Non-Selective Environment:The medium lacks inhibitory substances, allowing for the growth of a broad range of bacteria.
- Buffering System:The phosphate buffer system protects bacteria from detrimental changes in pH, ensuring their viability.
- Nutrient-Rich:Proteose peptone provides the necessary carbon, nitrogen, vitamins, and minerals for bacterial growth and repair.
- Compliance with Standards:The medium's specifications align with international standards such as ISO 6579-1:2017 for Salmonella detection and ISO 22964 for Enterobacteriaceae from milk products.
- Food Microbiology:It serves as a pre-enrichment medium for detecting Salmonella and other Enterobacteriaceae in food samples.
- Clinical Samples:BPW can also be used for the preliminary isolation and recovery of injured bacteria from clinical specimens.
Casein Soyabean Digest Broth (Soyabean Casein Digest Medium)
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₹ 2125 / Kg Get Latest Price
Product Brochure
| Packaging Size | Plastic Bottle |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Type | 500g |
| Country of Origin | Made in India |
| Product Code | MH011 |
| Pack Size | 500g |
| Appearance | Cream to yellow, homogeneous free-flowing powder |
| Ingredient | Concentration (g / L) |
| ancreatic digest of casein (Tryptone) | 17.00 |
| Papaic digest of soybean (Soy peptone) | 3.00 |
Minimum order quantity: 1 Kg
Casein Soyabean Digest Broth, also known as Tryptic Soy Broth (TSB) or Caso Broth, is a highly nutritious, general-purpose liquid medium used extensively in microbiology for the cultivation of a wide variety of microorganisms, including bacteria and fungi. It is widely recommended by various pharmacopoeias (USP, EP, JP, BP, IP) for sterility testing and microbial limit testing of pharmaceutical products and raw materials. Key Details and CompositionThe medium is a clear, light yellow solution that provides a rich source of nutrients, fostering robust microbial growth. The key ingredients and their functions are:
- Pancreatic Digest of Casein and Papaic Digest of Soyabean Meal: These enzymatic digests are the primary nutrient sources, providing essential amino acids, nitrogenous compounds, and long-chain peptides necessary for microbial growth.
- Dextrose (Glucose): A fermentable carbohydrate that serves as a carbon and
Nutrient Agar Plate Himedia
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₹ 1435 / Piece Get Latest Price
Product Brochure
| Packaging Size | 20PT |
| Brand | Himedia |
| Usage/Application | Laboratory |
| Color | Light Amber coloured medium - Sterile medium in 90mm disposable petri plates |
| Country of Origin | Made in India |
| Physical state | solid |
| Pack Size | 20PT |
| Recommended storage temperature | 20–30 °C |
| pH (value) | 7,2–7,6 |
Minimum order quantity: 1 Piece
A HiMedia Nutrient Agar Plate is a sterile, ready-to-use petri dish containing a general-purpose microbiological growth medium. It is used for cultivating a wide variety of non-fastidious microorganisms, for purity checks before further testing, and is also suitable for teaching and demonstrations. The medium typically consists of peptic digest of animal tissue, sodium chloride, beef extract, yeast extract, and agar, with a pH that is generally maintained around 7.2-7.6. Key features
- Form: Pre-poured, sterile plates with a solid growth medium.
- Purpose: A general-purpose medium for routine cultivation of a wide range of microorganisms, particularly non-fastidious ones.
- Common uses:
- Maintaining microbial cultures.
- Purity checking prior to biochemical or serological testing.
- Microbiological examination of water, dairy products, and other materials.
- Educational and teaching purposes.
- Composition: Contains a balanced mix of nutrients like peptic digest of animal tissue, beef extract, and yeast extract, with sodium chloride to maintain osmotic balance and agar as a solidifying agent.
- Storage: Should be stored between 15-25°C.
- Enrichment: Can be enriched with additives like serum or blood to cultivate more fastidious organisms.
Luria Bertani Agar Plate Himedia
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₹ 1695 / Piece Get Latest Price
Product Brochure
| Packaging Size | 20PT |
| Brand | Himedia |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Pack Size | 20PT |
| Recommended Storage Temperature | Store between 20-30°C. |
| Appearance | Sterile medium in 90mm disposable petri plates Yellow to Amber coloured medium |
| pH | 7.30 - 7.70 |
Minimum order quantity: 1 Piece
A HiMedia Luria Bertani (LB) Agar plate is a nutrient-rich medium used for the cultivation of bacteria, especially recombinant strains of E. coli for genetic and molecular biology studies. It contains tryptone (casein enzymic hydrolysate), yeast extract, and sodium chloride to provide essential nutrients, maintain osmotic equilibrium, and support microbial growth. The agar component is what solidifies the medium into a plate for culturing. Key features and uses
- Composition: Made with casein enzymic hydrolysate (tryptone), yeast extract, sodium chloride, and agar.
- Purpose: Primarily used for cultivating and maintaining recombinant strains of E. coli for genetic and molecular studies. It can also be used for the routine cultivation of other non-fastidious microorganisms.
- Nutritional richness: The combination of tryptone and yeast extract provides peptides, peptones, and a Vitamin B complex, while sodium chloride ensures osmotic balance.
- Customization: The medium can be modified by adding other substances like antibiotics to select for specific strains or X-Gal for blue-white screening.
- Preparation: To prepare, suspend the powder in distilled water, heat to dissolve, sterilize via autoclaving (e.g., at 121°C for 15 minutes), and pour into sterile plates.
Sabouraud Dextrose Agar, Granulated Himedia
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₹ 4565 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Packaging type | HDPE |
| Pack Size | 500g |
| Product Type | Granulated |
| Physical Form | Granular |
| Recommended Storage Temperature | On receipt store between 10-30°C |
| Specific end uses | Apart from the uses mentioned in section 1.2 no other specific uses are stipulated |
| Appearance | Cream to yellow coloured granular medium |
| pH | 5.40 - 5.80 |
Minimum order quantity: 1 Kg
HiMedia's Sabouraud Dextrose Agar, Granulated is a microbiology medium used for isolating and cultivating fungi like yeasts and molds, especially those associated with skin infections. Its low pH (around 5.6) and high dextrose concentration inhibit the growth of most bacteria, making it selective for fungi. The granulated form offers ease of handling, dissolution, and storage compared to traditional powdered media. Key features
- Selective growth: The medium's acidic pH and high sugar content are specifically designed to suppress bacterial growth, allowing fungi like yeasts, molds, and dermatophytes to thrive.
- Wide applications: It is used in both clinical settings and for general microbiology to cultivate fungi from various specimens, including those from skin, and can also be used to test antimycotic substances.
- Granulated form: The granulated format ensures quick and complete dissolution, better stability, and is easy to handle and weigh.
- Supports aciduric organisms: Besides fungi, it also supports the growth of acid-tolerant (aciduric) microorganisms.
- Inhibits bacteria: The low pH (around 5.6) is a key feature that prevents the growth of most bacteria in the sample.
- For isolation from contaminated samples, consider using a selective medium simultaneously with Sabouraud Dextrose Agar.
- Incubate cultures for an extended period (up to 4-6 weeks) before reporting them as negative for fungal growth, especially for dermatophytes.
MYP Agar Base Phenol Red Egg Yolk Polymyxin Agar Base) Himedia
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₹ 5275 / Gram Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Product Code | M636 |
| Pack Size | 500g |
| CAS NO | 143-74-8 |
| PH | 6-90-7.30 |
Minimum order quantity: 1 Gram
Himedia's MYP Agar Base is used for the selective isolation and identification of Bacillus cereus and pathogenic Staphylococci. The medium uses Phenol Red to indicate mannitol fermentation (yellow colonies), egg yolk to reveal lecithinase activity (white precipitate zones), and Polymyxin B Sulfate to inhibit Gram-negative bacteria. Purpose
- Isolate and identify pathogenic Staphylococci and Bacillus species.
- Differentiate Bacillus cereus from other Bacillus species.
- Peptone and Meat Extract:Provide nitrogen, carbon, vitamins, and minerals for bacterial growth.
- D-Mannitol:A carbohydrate source that, when fermented by bacteria, produces acid and lowers the pH.
- Phenol Red:A pH indicator that turns colonies yellow when mannitol is fermented, indicating a mannitol-fermenting organism.
- Egg Yolk Emulsion:A differential component that produces a white precipitate zone around lecithinase-producing colonies, such as Staphylococcus aureus and some Bacillus species.
- Polymyxin B Sulfate:A selective agent that restricts the growth of Gram-negative bacteria, like E. coli and Pseudomonas aeruginosa.
- Agar:The solidifying agent that provides the gel-like consistency for the medium.
- Yellow colonies with a white precipitate zone:Indicates a mannitol-fermenting, lecithinase-producing organism (e.g., Staphylococcus aureus).
- Yellow colonies without a white precipitate zone:Suggests a mannitol-fermenting, lecithinase-negative organism (e.g., Bacillus subtilis).
- No yellow color with a white precipitate zone:Indicates a non-mannitol-fermenting, lecithinase-positive organism (e.g., Bacillus cereus).
Yeast Glucose Chloramphenicol Agar
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₹ 5525 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Packaging type | HDPE |
| Pack Size | 500g |
| Physical Form | Powder |
| Recommended Storage Temperature | On receipt store between 15-25°C |
| Specific end uses | Apart from the uses mentioned in section 1.2 no other specific uses are stipulated |
| Appearance | Cream to yellow coloured homogeneous free flowing powder |
| pH | 6.40 - 6.80 |
Minimum order quantity: 1 Kg
Yeast Glucose Chloramphenicol Agar is a selective culture medium from HiMedia for enumerating yeasts and moulds. It is recommended for use in milk and milk products. The medium contains glucose as a carbohydrate source and chloramphenicol to inhibit bacterial growth, allowing for the selective growth of fungi.
- Primary use: Selective cultivation of yeasts and moulds.
- Specific application: Recommended by organizations like the American Public Health Association (APHA) and the International Dairy Federation (IDF) for enumerating yeasts and moulds in milk and milk products.
- Mechanism:
- Glucose: Provides a carbohydrate energy source for the fungi.
- Chloramphenicol: An antibiotic that suppresses the growth of accompanying bacteria, making the medium selective for fungi.
- Performance: After incubation, yeast and mould colonies can be distinguished based on their colony morphology.
Rappaport Vassiliadis Medium Himedia
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₹ 5790 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Packaging type | HDPE |
| Physical Form | Powder |
| Recommended Storage Temperature | On receipt store between 10-30°C |
| Specific end uses | Apart from the uses mentioned in section 1.2 no other specific uses are stipulated |
| Appearance | Light yellow to light blue coloured Page 4 of 7 homogeneous free flowing powder. |
| pH | 4.80 - 5.20 |
| Soy peptone | 4.5 g |
| Sodium chloride | 8.0 g |
| Potassium dihydrogen phosphate | 1.6 g |
| Dipotassium hydrogen phosphate | 0.4 g |
| Magnesium chloride (anhydrous) | 13.58 g |
| Malachite green | 0.036 g |
| Final pH (at 25 °C) | 5.2 ± 0.2 |
| Pack Size | 500g |
Minimum order quantity: 1 Kg
Rappaport Vassiliadis (RV) Medium by HiMedia is a selective enrichment broth used for isolating Salmonella species from various samples like food, environmental sources, and clinical specimens. It works by creating conditions that are toxic to most bacteria, such as low pH, high osmotic pressure from magnesium chloride, and the presence of malachite green, while allowing Salmonella to thrive. The medium is also used for testing pharmaceutical products according to pharmacopeia guidelines. Key features and description
- Purpose: To selectively enrich and promote the growth of Salmonella while inhibiting the growth of other bacteria.
- Composition: It contains ingredients that create a selective environment:
- Soya peptone: Provides essential nutrients.
- Magnesium chloride: Increases osmotic pressure.
- Malachite green: Inhibits the growth of most other bacteria, especially gram-positive ones.
-
- Potassium and sodium phosphates: Buffer the medium to maintain a stable pH.
- Sodium chloride: Helps maintain osmotic balance.
- Incubation: The standard incubation temperature is 42−43∘C42 minus 43 raised to the composed with power cap C42−43∘𝐶.
- Procedure: After incubation, the enriched culture is subcultured onto a selective agar medium, such as MacConkey Agar or XLD Agar, for isolation and identification.
- Applications:
- Enrichment of Salmonella from food and environmental samples.
- Isolation of Salmonella from clinical specimens, including feces.
- Testing pharmaceutical products according to USP/EP/BP/JP harmonized methodologies.
StabiFlexiLoop Plus 4 Himedia
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₹ 985 / Piece Get Latest Price
Product Brochure
| Packaging Size | 1x100NO |
| Grade | LR (Laboratory) |
| Brand | HiMedia |
| Usage / Application | laboratory |
| Packaging Type | Box |
| Country of Origin | India |
| Handling | Refer to Section 8 |
| Storage | Store below 30°C |
| Sterilized | Ready for use in a sterile environment. |
| Flexible | Allows for uniform streaking of cultur |
| Calibrated | Specifically calibrated to a volume of \(0.01\) ml for consistent inoculation. |
| Double-sided | Has both a loop and a pointed end, suitable for streaking and stabbing. |
| Individually sealed | Each loop is packaged separately to maintain sterility. |
| Smooth surface | Ensures easy and efficient streaking without damaging the media. |
| Design | Flexible to facilitate streaking but rigid enough for subsurface inoculation |
| Features | Stabilizer tip |
| Product Code | PW053 |
| Loop diameter | 4.40 mm |
| Volume calibration | Each loop is calibrated to 0.01 mL |
| Material | Inert polymer (plastic) — suitable for microbiological / lab use. |
Minimum order quantity: 1 Piece
The StabiFlexiLoop Plus - 4 is a disposable, sterilized, and flexible inoculation loop from HiMedia for microbiological work. Key features include its smooth surface for easy streaking, a built-in stabber tip, and color-coding for easy identification. The "- 4" in the name refers to a volume of 4μL4 mu cap L4 �. Key features
- Type: Disposable, sterilized inoculation loop.
- Application: Used for streaking microbial cultures on agar plates.
- Design: Has a smooth, flexible loop and a built-in stabber tip for inoculating both plates and broths.
- Volume: Calibrated to a specific volume, indicated by the "4" in the name.
- Identification: Color-coded for easy identification.
- Packaging: Sold in packs of 100 or 500 (5 x 100) individual loops.The "StabiFlexiLoop Plus 4" from HiMedia (SKU: PW053) is a disposable, sterile plastic inoculating loop for microbiology labs. Key specifications include its flexible yet rigid design for streaking and subsurface inoculation, a stabilizer tip, and its use in quantitative procedures like sampling and serial dilutions. The "4" likely refers to a specific loop size or design not detailed in the provided results.
- Product Type: Disposable plastic inoculating loop
- Brand: HiMedia
- SKU: PW053
- Design: Flexible to facilitate streaking but rigid enough for subsurface inoculation
- Features: Stabilizer tip
- Applications: Quantitative procedures (sampling, serial dilutions, urine counts), bacterial inoculation, microbiology, molecular biology, and plant tissue culture
- Availability: Available as an individual loop or in an economical pack of 100
LP Enrichment Supplement 1 Himedia
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₹ 9865 / Piece Get Latest Price
Product Brochure
| Packaging Size | 5-vl |
| Brand | Himedia |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Recommended Storage Temperature | On receipt store at -20 C |
| Appearance | Pale coloured opaque solution |
| Pack Size | 5vl |
Minimum order quantity: 1 Piece
HiMedia's LP Enrichment Supplement 1 (FD214) is a component for the selective differentiation of Listeria monocytogenes from other Listeria species, particularly for use with media like HiCrome™ Listeria Ottaviani-Agosti Agar Base. It contains L-phosphatidylinositol, which is hydrolyzed by the phosphatidylinositol-specific phospholipase C (PIPLC) enzyme produced by L. monocytogenes, forming an opaque halo around colonies to visually differentiate it from other Listeria species, according to the manufacturer's Technical Data Sheet. Intended Use:
- Selective differentiation of Listeria monocytogenes from other Listeria species.
- For use with media that detect PIPLC activity, such as HiCrome™ Listeria Ottaviani-Agosti Agar Base.
- L-phosphatidylinositol: The substrate that is hydrolyzed by L. monocytogenes' PIPLC enzyme.
- Distilled water: The solvent for the active ingredient.
- Listeria monocytogenes possesses the phosphatidylinositol-specific phospholipase C (PIPLC) enzyme.
- This enzyme hydrolyzes the L-phosphatidylinositol in the culture medium.
- The hydrolysis creates an opaque halo around the L. monocytogenes colonies, allowing for their visual identification.
- Add the sterile contents of one vial of LP Enrichment Supplement 1 (FD214) to a prepared base medium, such as an Ottaviani-Agosti Agar Base, after cooling to a suitable temperature.
- Mix well and pour into sterile Petri plates for use in bacterial culture.
Sabouraud Chloramphenicol Agar
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₹ 1165 / Kg Get Latest Price
Product Brochure
| Packaging Size | 100 g |
| Grade | AR (Analytical) |
| Brand | HiMedia |
| Physical State | Powder |
| Usage / Application | Laboratory |
| Packaging Type | Plastic Bottle |
| Country of Origin | India |
| Product Code | M1067 |
| Colour | Cream to yellow homogeneous free flowing powde |
| Component | Amount |
| D-Glucose | 40.0 g |
| Peptones (casein/meat peptone) | 10.0 g |
| Agar | 15.0 – 20.0 g |
| Chloramphenicol | 0.05 g (50 mg) |
| Final pH | 5.6 ± 0.2 at 25°C |
Minimum order quantity: 1 Kg
Sabouraud Chloramphenicol Agar is a specialized culture medium designed to selectively grow yeasts and molds (fungi) while inhibiting bacteria, making it ideal for isolating pathogenic fungi from clinical or food samples. Its key features are a nutrient-rich base (Sabouraud's agar) with high dextrose for fungal energy and low pH to favor fungi, plus the antibiotic Chloramphenicol to suppress most contaminating bacteria, allowing fungi to flourish and often produce pigments or spores for identification. Key Components & Function:
- Sabouraud Dextrose Agar (SDA): Provides carbohydrates (dextrose for energy) and peptones (nitrogen/amino acids) for fungal nutrition.
- Dextrose (Glucose): High concentration promotes fungal growth and spore/pigment formation.
- Low pH (Acidic): Inhibits many bacteria while favoring fungi.
- Chloramphenicol: An antibiotic that inhibits a broad spectrum of Gram-positive and Gram-negative bacteria, making the medium selective.
- Culturing fungi from skin, nail scrapings, and other clinical samples.
- Microbiological testing of non-sterile food and dairy products.
- Studying fungal morphology, including spore and pigment production.
- Typically a dehydrated powder that forms a yellowish to amber, slightly hazy medium when prepared.
- Plates should be free of visible moisture before use.
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