Himedia
Providing you the best range of cefepime 30mcg 1vl himedia, r2a agar himedia, lysine iron cystine broth base, sabouraud dextrose broth, diagnostic stuart s urea broth base and sabouraud chloramphenicol agar with effective & timely delivery.
Cefepime 30mcg 1Vl Himedia
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₹ 265 / Piece Get Latest Price
Product Brochure
| Packaging Size | 1VL |
| Brand | Himedia |
| Packaging Details | Bottle |
| Packaging Type | Vial |
| Color | White |
| Country of Origin | Made in India |
| Usage/Application | Laboratory |
| Product Code | SD219-1VL |
Minimum order quantity: 1 Piece
Cefepime 30mcg 1VL by HiMedia refers to filter paper discs containing cefepime, a fourth-generation cephalosporin antibiotic, at a concentration of 30 micrograms per disc. These discs are used in antimicrobial susceptibility testing, specifically for the Kirby-Bauer method, to assess the effectiveness of cefepime against various bacterial cultures. Each disc is 6mm in diameter and printed with "CPM 30" on both sides for easy identification. Key features:
- Antimicrobial susceptibility testing: These discs are designed for determining the sensitivity of bacteria to cefepime.
- Kirby-Bauer method: The discs are used in the standardized Kirby-Bauer method, a common technique for antibiotic susceptibility testing.
- 30mcg concentration: Each disc contains a precise amount of cefepime (30 micrograms).
- Visual identification: The discs are marked with "CPM 30" on both sides for easy recognition.
- Storage: Cefepime discs should be stored at -20°C under dry conditions, ideally with a desiccant.
R2A Agar HIMEDIA
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₹ 5115 / Piece Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | Microbiological Grade |
| Form | Powder |
| Brand | HIMEDIA |
| Prescription/Non prescription | Prescription |
| Product Code | M962 |
| Packaging type | HDPE |
Minimum order quantity: 500 Piece
R-2A Agar is a low-nutrient culture medium used to count bacteria in treated drinking water:
- Description: A solid medium that's clear to slightly turbid and yellowish in color. It has a pH value of 7.0–7.4 at 25°C.
- Purpose: Used to count bacteria in drinking water, and to recover stressed or chlorine-tolerant bacteria.
- Development: Developed by Reasoner and Geldreich.
- Incubation: Incubate aerobically at 32.5 ± 2.5°C for up to 3 days for bacteria, and at 22.5 ± 2.5°C for 5–7 days for yeasts and molds.
- Quality control: Available in basic, plus, or prime quality control levels.
- Shelf life: Can have a shelf life of up to five years.
- Safety: Not classified as dangerous, but it's recommended to consult the safety data sheet for correct use.
- Intended use: For professional use only, by properly trained operators.
Lysine Iron Cystine Broth Base
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₹ 4485 / Piece Get Latest Price
Product Brochure
| Grade | Microbiology Grade |
| Packaging Size | 500G |
| Brand | Himedia |
| Packaging Details | Bottle |
| Usage/Application | Laboratory Chemical |
| Category | HIMEDI |
| Country of Origin | Made in India |
Minimum order quantity: 5 Piece
Lysine Iron Cystine Broth is a medium used to quickly detect Salmonella in food, food ingredients, and feed materials:
- Purpose: It's a modification of the Hoben, Aston, and Peterson formula, and can detect Salmonella in food samples in as little as three days.
- Ingredients: It contains casein enzymic hydrolysate and L-Cystine.
- Suppliers: Some suppliers of Lysine Iron Cystine Broth include:
- HiMedia: Supplies Lysine Iron Cystine HiVeg Broth Base, which is prepared using HiVeg hydrolysate
- CDH Fine Chemical: An ISO certified manufacturer, supplier, and exporter of Lysine Iron Cystine MiVeg™ Broth Base
- MyMicroLab: Sells Lysine Iron Broth Base online, along with other lab chemicals and culture media
Sabouraud Dextrose Broth
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₹ 2780 / Bottle Get Latest Price
Product Brochure
| Media Type | Liquid Medium (Broth). |
| Packaging Size | 500 g |
| Packaging Details | Plastic Bottle |
| Brand | Himedia |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Pack Size | 500G |
| Product Coce | MH033 |
Minimum order quantity: 1 Bottle
Sabouraud Dextrose Broth (SDB) is a liquid microbiological medium primarily used for the cultivation of fungi, including yeasts and molds, from various samples. It is a modified version of Dextrose Agar, initially described by Sabouraud. SDB is particularly useful because its low pH inhibits the growth of most bacteria, while promoting the growth of fungi. Here's a more detailed description:Key Characteristics:
- Selective:The low pH (typically 5.6 ± 0.2) inhibits most bacteria, making it ideal for isolating fungi from samples that may be contaminated with bacteria.
- Nutrient Rich:SDB contains peptones (from casein and animal tissue) and dextrose (glucose) which provide the necessary nitrogen, carbon, and energy source for fungal growth.
- Versatile:SDB is used for cultivating a wide range of fungi, including dermatophytes (fungi that cause skin infections) and other yeasts and molds.
- Standardized:SDB is often specified in pharmacopoeias like USP, EP, and JP, indicating its use in the microbiological examination of non-sterile products.
- Applications:SDB is used in various applications such as:
- Microbiological testing of pharmaceutical products
- Isolation and cultivation of fungi from clinical and non-clinical samples
- Testing antifungal agents
- Detection of Candida albicans in non-sterile products
- Preparation:Typically, SDB is prepared by dissolving the dehydrated powder in distilled or purified water and autoclaving to sterilize.
- Storage:SDB is typically stored at room temperature (15-25°C).
Diagnostic Stuart s Urea Broth Base
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₹ 5090 / Piece Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Make | Himedia |
| Form | Powder |
| Packing Type | Bottle |
| CAS No | 143-74-8 |
Minimum order quantity: 1 Piece
Diagnostic Stuart's Urea Broth Base, also known as Urea Broth Base, is a specialized medium used to detect urease production in bacteria. It's particularly useful for differentiating Proteus species from other enteric bacilli. Urease is an enzyme that some bacteria produce to break down urea, releasing ammonia and causing the medium to become alkaline. A positive result, indicated by a color change from yellow to pink or red due to the pH indicator phenol red, signifies urease production. Here's a more detailed breakdown:
- Urease Test:This medium is used to test for the presence of urease, an enzyme that some bacteria produce to hydrolyze urea.
- Urea Utilization:Bacteria with urease produce ammonia from urea, which raises the pH of the medium and causes a color change in the indicator.
- Differentiation:Stuart's Urea Broth Base is helpful in distinguishing Proteus species, which are typically urease-positive, from other enteric bacteria like Salmonella and Shigella, which are often urease-negative.
- Principle:The broth contains urea, a substrate for urease, and a pH indicator like phenol red. If a urease-positive organism is present, it will break down urea, releasing ammonia and raising the pH, which is indicated by the color change in the phenol red.
- False Positives:Protein hydrolysis and excess amino acids can also lead to alkalinity and false positive reactions, so a control medium without urea is recommended.
- Commercial Availability:This broth is commercially available in various forms, including dehydrated powder and prepared tubes
Sabouraud Chloramphenicol Agar
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₹ 5240 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Final pH | 5.40 - 5.80 |
| Product Code | M1067 |
| CAS Number | 56-75-7 |
| Country of Origin | Made in India |
| Form | Powder |
| Make | Himedia |
| Usage | Laboratory Chemical |
Minimum order quantity: 1 Kg
Sabouraud Chloramphenicol Agar (SCA) is a selective medium used for cultivating yeasts and molds, particularly those associated with skin infections. The medium's acidic pH inhibits bacterial growth while promoting fungal growth, and the addition of chloramphenicol further inhibits bacteria. Key features of Sabouraud Chloramphenicol Agar:
- Selective Medium:SCA is designed to favor the growth of fungi while inhibiting bacterial growth.
- Acidic pH:The low pH (typically around 5.6) is a key factor in inhibiting bacterial growth.
- Chloramphenicol:This antibiotic further restricts bacterial growth, making the medium more selective for fungi.
- Applications:SCA is used for isolating and culturing pathogenic fungi, particularly those involved in skin and other infections, and for the isolation of dermatophytes.
- Composition:The basic medium is typically Sabouraud Dextrose Agar, and chloramphenicol is added as an antibiotic.
Blood Agar Base 500g Himedia M073
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₹ 4270 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Manufacturer | HIMEDIA |
| Brand | HiMedia |
| Composition | HM peptone B,Tryptose,Sodium Chloride |
| Prescription/Non prescription | Prescription |
| Final pH | 7.3 pH |
| Storage Temperature | 25 DegreeC |
| Product Code | M073 |
Minimum order quantity: 2 Bottle
Generally used as a basal medium for preparing blood agar by supplementation with blood. It can also be used as general-purpose media without the addition of blood. It can be used with added phenolphthalein phosphate for the detection of phosphate producing Staphylococci, with added salt and agar for assessment of surface contamination on equipment and to determine salinity range of marine Flavobacteria. It can also be used for preparation of Salmonella Typhi antigens, recommended by APHA for testing of food samples.
Lysine Iron Agar HIMEDIA
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₹ 4010 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Manufacturer | HIMEDIA |
| Composition | Peptone,Yeast Extract,L-Lysine,Sodium Thiosulphate |
| Prescription/Non prescription | Prescription |
| Final pH | 6.7 pH |
| Storage Temperature | 25 DegreeC |
| Product Code | M377 |
| Brand | HIMEDIA |
Minimum order quantity: 2 Bottle
Lysine Iron Agar is recommended for differentiation of enteric organisms especially Salmonella arizonae, based on their ability to decarboxylate or deaminate lysine and to form hydrogen sulphide (Hâ‚‚S). Dextrose is a source of fermentable carbohydrate. Ferric ammonium citrate and sodium thiosulphate are indicators of H2S formation. Cultures that produce hydrogen sulphide cause blackening of the medium due to ferrous sulphide production. Lysine decarboxylation causes an alkaline reaction (purple colour) to give the amine cadaverine and the organisms which do not decarboxylate lysine, produce acid butt (yellow colour).
CLED Agar Bromo Thymol Blue HIMEDIA
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₹ 5050 / Piece Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Brand | HiMedia |
| Packaging Type | Bottle |
| Category | Culture Media |
| Physical State | Powder |
| Purity | 99% |
| Composition | Peptone,Tryptone,HM Peptone B,Lactose |
| Treatment | Beef Extract |
| Prescription/Non prescription | Prescription |
| Final pH | 7.3 pH |
Minimum order quantity: 2 Piece
C.L.E.D. Agar w/Bromo Thymol Blue is recommended for use in urinary bacteriology. This medium promotes the growth of all urinary pathogens. Lactose is the fermentable sugar. Bromo thymol blue is the pH indicator which turns yellow at acidic pH. Colonies grow as blue or yellow due to fermentation of lactose. HiMedia M792 is C.L.E.D. (Cystine Lactose Electrolyte Deficient) Agar with Bromo Thymol Blue, a microbiological medium used to isolate, count, and differentiate urinary pathogens by detecting lactose fermentation (yellow colonies for fermenters, blue/purple for non-fermenters). Its key specs include a composition of peptones, tryptone, lactose (10g/L), L-cystine (0.128g/L), bromothymol blue (0.02g/L), and agar (15g/L), with a final pH of 7.3 ± 0.2 at 25°C, appearing green when prepared. Key Specifications:
- Product Name: C.L.E.D. Agar w/ Bromo Thymol Blue
- Product Code: M792
- Intended Use: Isolation, enumeration, and identification of urinary pathogens.
- Appearance (Powder): Cream to yellow, homogeneous, free-flowing.
- Appearance (Prepared Medium): Green, clear to slightly opalescent gel.
- pH (Final): 7.3 ± 0.2 (at 25°C).
- Composition (per Liter):
- Peptone: 4.0 g
- Tryptone: 4.0 g
- HM Peptone B: 3.0 g
- Lactose: 10.0 g
- L-Cystine: 0.128 g
- Bromothymol Blue: 0.02 g
- Agar: 15.0 g
- Directions: Suspend 36.15g in 1L water, boil to dissolve, sterilize (121°C, 15 min), cool to 45-50°C, pour plates.
- Storage: Store at 10-30°C in a tightly closed container; keep dry.
- Lactose Fermentation: Lactose is fermented by some bacteria, producing acid which turns the bromothymol blue indicator yellow.
- Cystine: Supports the growth of dwarf coliforms, crucial for urinary diagnostics.
- Appearance of Colonies: Blue/purple (non-fermenters like Proteus) or yellow (lactose fermenters like E. coli).
Soyabean HiVeg Medium Sterile Powder
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Ready-to-use: Convenient sterile powder form for easy preparation.
Nutrient-rich: Supports the growth of a wide variety of microorganisms.
Plant-based: Made from vegetable sources, suitable for various dietary preferences.
Versatile application: Ideal for microbiological culture media in laboratories.
Reliable: Consistent performance for accurate and reproducible results.
Easy storage: Long shelf life when stored properly.
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Himedia MV011G is Soyabean HiVeg™ Medium (Tryptone Soya Broth), a highly nutritious, animal-free, general-purpose culture medium used in microbiology, especially for pharmaceutical sterility testing, providing essential nutrients (peptones, dextrose, salts) for diverse microorganisms, with a standard pH of 7.3±0.2 and yellow, clear appearance when prepared, available in dehydrated or ready-to-use sterile forms. Key Specifications:
₹ 3609 / Gram Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Manufacturer | HIMEDIA |
| Composition | Nutrient-rich: Supports the growth of a wide variety of microorganisms. |
| Prescription/Non prescription | Prescription |
| Product Code | MV011G-500G |
| Brand | HIMEDIA |
| CAS Number | 9002-18-0 |
| Shelf Life | 36 MONTH |
Minimum order quantity: 1 Gram
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Himedia MV011G is Soyabean HiVeg™ Medium (Tryptone Soya Broth), a highly nutritious, animal-free, general-purpose culture medium used in microbiology, especially for pharmaceutical sterility testing, providing essential nutrients (peptones, dextrose, salts) for diverse microorganisms, with a standard pH of 7.3±0.2 and yellow, clear appearance when prepared, available in dehydrated or ready-to-use sterile forms. Key Specifications:
- Product Type: Soyabean HiVeg™ Medium (Tryptone Soya Broth/SCDM).
- Purpose: Supports growth of wide range of microorganisms, used for sterility testing (Pharma, Clinical), and microbial limit tests.
- Composition (g/L): HiVeg hydrolysate (17.0), Soya peptone (3.0), Sodium Chloride (5.0), Dipotassium Hydrogen Phosphate (2.5), Dextrose (2.5) (for sterile powder).
- pH (Final): 7.3 ± 0.2 at 25°C.
- Appearance (Powder): Cream to yellow granular/homogeneous powder, may have green tinge.
- Appearance (Prepared): Light amber to yellow clear solution/gel.
- Directions: Suspend 30g in 1000ml water; heat to dissolve (if dehydrated); sterilize by autoclaving (121°C, 15 psi, 15 min) or use as is if sterile powder.
- Features: Animal-free (BSE/TSE risk-free), provides amino acids, peptides, carbon, and ions.
- MV011G: Sterile Powder (Ready-to-use).
- GMV011G: Granulated/Dehydrated (Requires preparation).
Soybean Casein Digest Medium
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₹ 2095 / Piece Get Latest Price
Product Brochure
| Physical State | Powder |
| Packaging Details | Plastic Bottle |
| Packaging Size | 500G |
| Usage/Application | Laboratory/pharmaceutical or R&D |
| Color | Yellow |
| Brand | Himedia |
| Type | Bottle |
| Country of Origin | Made in India |
| Final pH | 7.10-7.50 |
| Storage | 10-30 C |
Minimum order quantity: 1 Piece
Soyabean Casein Digest Medium (SCDM), also known as Tryptone Soya Broth (TSB), is a widely used general-purpose medium for cultivating a broad range of bacteria and fungi. It's specifically formulated to provide a rich source of nutrients for microbial growth, making it suitable for various applications like sterility testing, microbial limit testing, and general laboratory cultivation. Key Features and Uses:
- Nutrient Rich:SCDM provides essential nutrients derived from soyabean and casein digests, supporting the growth of diverse microorganisms.
- Versatile:Used for isolating, cultivating, and identifying a wide variety of organisms, including both fastidious and non-fastidious bacteria and fungi.
- Sterility Testing:Often used for sterility testing to detect contamination with low incidence fungi and aerobic bacteria.
- Microbial Limit Testing:Employed in microbial limit tests, ensuring the absence of microorganisms within specified limits.
- Preparation of Dilutions:Used to prepare dilutions of organisms for colony counts and standard inocula for susceptibility testing.
- Soybeanmeal digest: Provides a complex source of amino acids and nutrients derived from soybean.
- Casein digest: Enzymatically digested casein, offering another source of amino acids and peptides.
- Dipotassium phosphate: Acts as a buffer and nutrient.
- Sodium chloride: Helps maintain osmotic balance.
- Glucose: Provides a carbohydrate source for energy.
- Microbiology:Widely used in microbiology laboratories for growing various bacterial and fungal strains.
- Sterility Testing:A key component in sterility testing procedures, particularly in pharmaceutical and food industries.
- Microbial Limit Tests:Used in microbial limit tests to ensure the safety and quality of products.
- Cosmetics:Used for total aerobic microbial count and antimicrobial preservative effective tests in cosmetics.
R2A Agar Himedia
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₹ 5115 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 20 Kg |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory/Pharmaceuticals or R&D |
| Final pH | 7.0 - 0.2 |
| Color | Yellow |
| Brand | Himedia |
| Product Code | for analytical purposes for extraction |
| Country of Origin | Made in India |
| Shelf Life | 36 Months |
| Colour | Light Yellow |
| Solubility | 18.12 gm |
| Relevant identified uses | Laboratory Chemicals, Analytical Purpose, Biochemical Analysis |
| HSN Code | 38210000 |
Minimum order quantity: 1 Bottle
R2A Agar is a culture medium used for cultivating bacteria, especially those found in potable water, including those that are stressed or chlorine-tolerant. It's a low-nutrient medium that allows a wide range of bacteria to grow without fast-growing species outcompeting slow-growing ones. This makes it useful for recovering bacteria that might not thrive on richer media. Key Characteristics and Uses:
- Low Nutrient Content:R2A Agar has a lower concentration of nutrients compared to richer media, like Plate Count Agar.
- Slow-Growing Bacteria:It's designed to support the growth of bacteria that are slow-growing or stressed, which might be suppressed on richer media.
- Water Microbiology:It's particularly useful for monitoring heterotrophic bacterial populations in water treatment processes and distribution systems.
- Stressed Bacteria:It can help in the recovery of stressed or injured bacteria that may not grow well on high-nutrient media.
- Incubation Conditions:It's often incubated at 20-28°C for 5-7 days to encourage the growth of stressed and chlorine-tolerant bacteria.
Antibiotic Assay Medium No 35
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₹ 5515 / Bottle Get Latest Price
Product Brochure
| Physical State | Powder |
| Packaging Details | Plastic Bottle |
| Packaging Size | 500gm |
| Usage/Application | Laboratory /Microbiological or R&D |
| Brand | Himedia |
| Country of Origin | Made in India |
| Packing Size | 500G |
| Product Code | M798 |
| CAS NO | 7785-87-7 |
Minimum order quantity: 1 Bottle
Antibiotic Assay Medium No. 35 is a specialized culture medium used for the microbiological assay of bleomycin, specifically using Mycobacterium smegmatis as the test organism. It's formulated to support the growth of this bacteria while also allowing for the detection of bleomycin activity. This medium is often used as a base agar for diffusion assays. Key Features and Purpose: Bleomycin Assay:The primary purpose is to determine the potency of bleomycin, a chemotherapy drug. Test Organism:Mycobacterium smegmatis is the chosen organism due to its sensitivity to bleomycin. Formulation:The medium's composition, including the peptones, beef extract, sodium chloride, and agar, is designed to support the growth of the
Antibiotic Assay Medium No 35
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₹ 5515 / Piece Get Latest Price
Product Brochure
| Physical State | Powder |
| Packaging Details | Plastic Bottle |
| Packaging Size | 500gm |
| Usage/Application | Laboratory/R&D |
| Color | Medium amber |
| Brand | Himedia |
| Country of Origin | Made in India |
| Product Code | M798 |
| Packing Size | 500G |
| Appearance | Cream to yellow homogeneous free flowing powder |
| PH | 6.80-7.20 |
Minimum order quantity: 1 Piece
Antibiotic Assay Medium No. 35 is a specialized culture medium used for the microbiological assay of bleomycin, specifically using Mycobacterium smegmatis as the test organism. It's formulated to support the growth of this bacteria while also allowing for the detection of bleomycin activity. This medium is often used as a base agar for diffusion assays. Key Features and Purpose:
- Bleomycin Assay:The primary purpose is to determine the potency of bleomycin, a chemotherapy drug.
- Test Organism:Mycobacterium smegmatis is the chosen organism due to its sensitivity to bleomycin.
- Formulation:The medium's composition, including the peptones, beef extract, sodium chloride, and agar, is designed to support the growth of the Mycobacterium smegmatis.
- Glycerol:Glycerol is included as a carbon source, essential for the metabolism of the test organism.
- Base Agar:In diffusion assays, this medium serves as the base agar layer where the test sample is placed, allowing the antibiotic to diffuse into the medium and inhibit the growth of the Mycobacterium smegmatis.
Antibiotic Assay Medium No 11
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₹ 5330 / Piece Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Color | Cream to yellow |
| Country of Origin | Made in India |
| Product Code | MU004 |
| Packing Size | 500G |
| PH | 8.20-8.40 |
| Storage | 10-30 C |
Minimum order quantity: 1 Piece
Antibiotic Assay Medium No. 11, also known as Neomycin, Erythromycin Assay Agar, is a specialized medium used for microbiological assays of antibiotics. It's designed to support the growth of specific test organisms while allowing for the measurement of antibiotic activity. The medium is particularly useful for assays of antibiotics like neomycin and erythromycin, and others. Key Features and Uses:
- Specific Antibiotic Assays:This medium is specifically designed for assays of various antibiotics, including neomycin, erythromycin, chlortetracycline, and others.
- Microbiological Assay:It's used in microbiological assays to determine the potency and activity of antibiotics.
- Test Organisms:It supports the growth of specific test organisms, such as Bacillus pumilis, Bacillus subtilis, and Micrococcus luteus, which are used to determine antibiotic activity.
- pH Control:The medium is typically prepared to have a pH of 8.3 +/- 0.2.
- Seed Agar:It can also be used as a seed layer or base layer in various assay procedures.
- Preparation:The medium is prepared according to specific instructions, typically involving adding the dehydrated powder to distilled water and autoclaving.
- Inoculation:Test organisms are inoculated onto the agar surface after it has been cooled.
- Antibiotic Diffusion:Antibiotics are then introduced to the medium, and their diffusion through the agar is monitored for zones of inhibition, which indicate antibiotic activity.
EMB Agar Himedia
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₹ 895 / Gram Get Latest Price
Product Brochure
| Packaging Size | 100 g |
| Packaging Details | Plastic Bottle |
| Final pH | 25c |
| Product Code | M317 |
| Country of Origin | Made in India |
| Form | Powder |
| Usage | Laboratory/Microbiological or R&D |
| Make | Himedia |
Minimum order quantity: 1 Gram
EMB agar, specifically from HiMedia, is a microbiological culture medium used for the selective isolation and differentiation of Gram-negative bacteria, particularly enteric bacilli, from clinical and non-clinical samples. It contains lactose and sucrose as fermentable carbohydrates, and eosin Y and methylene blue as differential indicators. These indicators help distinguish between bacteria that can ferment lactose (and other sugars) and those that cannot. Key features and characteristics of HiMedia's EMB Agar:
- Selective: Inhibits the growth of Gram-positive bacteria, allowing primarily Gram-negative bacteria to grow.
- Differential: Eosin Y and methylene blue dyes differentiate bacteria based on their ability to ferment lactose and sucrose.
- Fermentable carbohydrates: Includes lactose and sucrose, which are metabolized by certain bacteria, producing acidic byproducts.
- Colony appearance:
- Lactose fermenters (e.g., coliforms): Typically produce purplish-black colonies, sometimes with a green metallic sheen, due to the absorption of the dye complex and a drop in pH.
- Non-lactose fermenters: Produce colorless or light pink colonies because they don't ferment the sugars and thus don't produce enough acid to cause the dyes to precipitate and be absorbed.
- Manufacturer: HiMedia Laboratories.
- Product code: M317.
- Application: Used in microbiology labs for isolating and identifying bacteria from various samples.
Vogel Johnson Agar Medium
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₹ 4270 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory/R&D |
| Color | White |
| Brand | Himedia |
| Country of Origin | Made in India |
| Product Code | MAP023 |
| Packing Size | 500G |
Minimum order quantity: 1 Bottle
Vogel-Johnson Agar (VJA) is a selective and differential medium used for the isolation and identification of Staphylococcus aureus, particularly coagulase-positive and mannitol-fermenting strains. It is known for its ability to differentiate S. aureus from other staphylococcal species. Key features of Vogel-Johnson Agar:
- Selective:The medium contains ingredients that inhibit the growth of many other bacteria, allowing S. aureus to flourish. These inhibitors include potassium tellurite, lithium chloride, and high glycine content.
- Differential:S. aureus colonies on VJA exhibit distinct characteristics: they appear black due to the reduction of tellurite, and they may be surrounded by a yellow zone due to mannitol fermentation, which acidifies the medium and turns the pH indicator, phenol red, yellow.
- Components:VJA includes ingredients like trytone and yeast extract (for nutrients), mannitol (for fermentation), potassium tellurite, lithium chloride, and glycine (for selective inhibition), phenol red (pH indicator), and dipotassium phosphate (buffer).
- Applications:It is used in microbial limit testing, for detecting staphylococcal carriers, and for health studies. It is also used in the food industry for isolating and enumerating S. aureus in food samples.
Violet Red Bile Glucose Agar
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₹ 4225 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Packaging Details | Plastic Bottle |
| Brand | Himedia |
| Product Code | MH581 |
| CAS Number | 548-62-9 |
| Country of Origin | Made in India |
| Usage/Application | Laboratory/Pharmaceuticals or R&D |
| Make | Himedia |
| Form | Powder |
Minimum order quantity: 1 Kg
Violet Red Bile Glucose Agar (VRBGA) is a microbiological growth medium used to isolate and enumerate bacteria belonging to the Enterobacteriaceae family, particularly in food and dairy products. It is a selective and differential medium that inhibits the growth of Gram-positive bacteria while allowing Gram-negative bacteria, especially those that ferment glucose, to grow and be identified. Key Features and Components:
- Selective Agents:Bile salts and crystal violet inhibit the growth of Gram-positive bacteria, while allowing Gram-negative bacteria to grow.
- Differential Agent:Glucose is the fermentable carbohydrate. Bacteria that ferment glucose produce acid, which lowers the pH of the medium. Neutral red, a pH indicator, turns red in acidic conditions, allowing for the identification of glucose-fermenting bacteria.
- Enrichment Components:Pancreatic digest of gelatin, yeast extract, and sodium chloride provide essential nutrients for bacterial growth.
- Solidifying Agent:Agar provides a solid surface for bacterial growth.
- 1. Inhibition:Bile salts and crystal violet inhibit the growth of most Gram-positive bacteria and some Gram-negative bacteria.
- 2. Fermentation:Enterobacteriaceae that can ferment glucose will produce acids, causing the neutral red indicator to turn red.
- 3. Colony Appearance:Glucose-fermenting bacteria will appear as red or reddish-purple colonies, often with a surrounding zone of bile precipitation (a reddish haze).
- Food Microbiology:VRBGA is used to detect and enumerate Enterobacteriaceae in various food products, including raw foods and processed foods.
- Dairy Microbiology:It is also used in the analysis of dairy products to assess the presence of coliform bacteria.
- Clinical Microbiology:VRBGA can be used in clinical settings to isolate and identify bacteria from clinical samples.
Soyabean Casein Digest Medium Tryptone Soya Broth
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₹ 2095 / Piece Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Product Code | M011 |
Soybean Casein Digest Medium (SCDM), also known as Tryptone Soya Broth (TSB), is a general-purpose medium used for cultivating a wide variety of microorganisms, including bacteria and fungi. It's known for its ability to support the growth of both fastidious and non-fastidious organisms. SCDM is also used in sterility testing and microbial limit testing. Key Characteristics and Uses:
- Versatile:SCDM can be used for a broad range of microbiological applications, including isolation, cultivation, and maintenance of microorganisms.
- Nutrient-Rich:The medium is designed to provide essential nutrients for microbial growth, including amino acids, long-chain peptides, and carbonaceous substances.
- Sterility Testing:It is recommended for sterility testing of various products, including pharmaceuticals and biological materials, as per various pharmacopeias.
- Microbial Limit Testing:SCDM is used for microbial limit testing to ensure the absence or low levels of microorganisms in products.
- Anaerobic Cultivation:Variations of SCDM, such as SCDM without dextrose, are specifically designed for culturing anaerobic microorganisms when the presence of carbohydrates is not desired.
- SCDM typically includes pancreatic digest of casein, papain digest of soybean meal, dextrose (glucose), sodium chloride, and dipotassium phosphate.
- The combination of these ingredients provides a rich source of nutrients, including amino acids, peptides, and vitamins, necessary for the growth of diverse microorganisms.
- SCDM is used as a broth medium, meaning it's a liquid medium used for cultivating microorganisms in liquid cultures.
- It can also be solidified by adding a solidifying agent like agar, creating a solid medium for isolating and identifying microorganisms on agar plates.
- SCDM is often used as a base medium for preparing more complex media for the cultivation of specific microorganisms.
Nutrient Agar Himedia
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₹ 815 / Kg Get Latest Price
Product Brochure
| Packaging Size | 100 g |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Product Code | M001 |
| Pack Size | 100 ML |
| HSN Code | 38220000 |
| Produced by | HiMedia Laboratories Private Limited |
Minimum order quantity: 1 Kg
Nutrient agar is a general-purpose growth medium used in microbiology to cultivate a wide variety of non-fastidious microorganisms, including bacteria and fungi. It's a solid medium, meaning it contains agar, which provides a solid support for microbial colonies. Here's a breakdown of its key aspects:Composition:
- Peptone: Provides a source of nitrogen, carbon, and amino acids.
- Beef extract: Offers additional nutrients, including vitamins and minerals.
- Yeast extract: Supplies B vitamins and other growth factors.
- Sodium chloride: Maintains the osmotic balance of the medium.
- Agar: A solidifying agent derived from seaweed, providing a stable surface for microbial growth.
- Cultivating microorganisms: It's a foundational medium for growing bacteria and other microbes, allowing researchers to study their characteristics and behavior.
- Clinical diagnostics: It's widely used in clinical labs to isolate and identify bacteria from patient samples.
- Research and teaching: It's a common tool for microbiology experiments and educational purposes.
- Food and water testing: It's used to assess the microbial content of food and water samples.
- Maintaining cultures: It can be used to preserve bacterial strains for future use.
- Non-selective:It supports the growth of a broad range of bacteria and fungi without favoring any particular type.
- Solid medium:The agar provides a solid surface for microbial colonies to grow, making them easier to observe and analyze.
- Nutrient-rich:The combination of peptone, beef extract, and yeast extract provides the essential nutrients for microbial growth
Reinforced Clostridial Agar
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₹ 5045 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Packaging Details | Plastic Bottle |
| Final pH | 6.60 - 7.00 |
| Brand | Himedia |
| Product Code | M154 |
| CAS Number | 52-89-1 |
| Country of Origin | Made in India |
| Usage/Application | Laboratory |
| Form | Powder |
| Packing Size | 500G |
| Packing Type | Bottle |
Minimum order quantity: 1 Kg
Reinforced Clostridial Agar (RCA) is a non-selective medium used for the cultivation and enumeration of Clostridia and other anaerobic bacteria. It is specifically designed to encourage the growth of Clostridia from small inocula and to achieve the highest viable cell counts. Key Features and Properties:
- Non-selective:RCA is a non-selective medium, meaning it does not contain ingredients that inhibit the growth of certain bacteria while favoring others.
- Enriched:It is enriched with nutrients, vitamins, and minerals to support the growth of Clostridia and other anaerobic bacteria.
- Used for:
- Cultivating Clostridia and other anaerobes.
- Enumerating Clostridia in various samples, including food and biological samples.
- Studies of spore-forming anaerobes, especially in dairy products.
- Microbiological examination of non-sterile products.
- Typical Formula:
- Contains peptones, yeast extract, dextrose, sodium chloride, soluble starch, and cysteine hydrochloride.
- Sodium acetate acts as a buffer.
- Principle:
- Peptic digest of animal tissue, beef extract, yeast extract, starch, and sodium acetate provide essential nutrients for bacterial metabolism.
- Cystine is a reducing agent.
- Sodium chloride maintains osmotic balance.
- Incubation:Incubation is typically performed anaerobically, meaning in an environment devoid of oxygen.
- Differential RCA:A differential version of RCA (DRCM) can be used to identify Clostridia that reduce sulphite, resulting in black colonies.
- Applications:Used in food microbiology, clinical microbiology, and other fields where the cultivation and enumeration of Clostridia and other anaerobes are required.
Mueller Hinton Agar Powder
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Mueller Hinton Agar (MHA) powder is a dehydrated, non-selective, solid culture medium primarily used for antimicrobial susceptibility testing, particularly the Kirby-Bauer disc diffusion method. It's known for its good batch-to-batch reproducibility and ability to support the growth of most non-fastidious bacterial pathogens. The powder is typically a cream to yellow, homogeneous, free-flowing powder. Key characteristics and uses:
₹ 4250 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Manufacturer | HIMEDIA |
| Packaging Details | Plastic Bottle |
| Composition | HM Infusion B From,Acicase,Starch,Agar |
| Treatment | Beef Infusion |
| Prescription/Non prescription | Prescription |
| Usage/Application | Laboratory |
| Final pH | 7.3 pH |
| Storage Temperature | 25 DegreeC |
| Color | Light Amber |
| Brand | Himedia |
| Product Code | M173 |
| Country of Origin | Made in India |
Minimum order quantity: 2 Bottle
Mueller Hinton Agar (MHA) powder is a dehydrated, non-selective, solid culture medium primarily used for antimicrobial susceptibility testing, particularly the Kirby-Bauer disc diffusion method. It's known for its good batch-to-batch reproducibility and ability to support the growth of most non-fastidious bacterial pathogens. The powder is typically a cream to yellow, homogeneous, free-flowing powder. Key characteristics and uses:
- Composition:MHA is a simplified medium containing beef extract, casein acid hydrolysate, starch, and agar.
- Nutrient-rich:It provides essential nutrients like amino acids, vitamins, and carbon sources for bacterial growth.
- Non-selective:It doesn't favor the growth of specific bacteria, allowing a wide range of common pathogens to grow.
- Antimicrobial Susceptibility Testing:It's the recommended medium for disc diffusion tests, where antibiotic-impregnated disks are placed on the agar to assess bacterial sensitivity.
- Kirby-Bauer Method:The standard method for using MHA involves applying a confluent lawn of bacteria to the agar surface and placing antibiotic discs.
- Standardized Method:MHA is a key component of standardized methods for antibiotic susceptibility testing, as it ensures consistent results.
- Appearance:The dehydrated powder should be cream to yellow, homogeneous, and free-flowing.
- Prepared Medium:After preparation, the agar should be light yellow to amber, clear to slightly opalescent.
- Sterility:Freshly prepared MHA should be sterile and free of any growth after incubation.
- Dehydrated Powder: Store below 30°C in a tightly closed container.
- Prepared Medium: Store at 2-8°C.
Chromogenic Coliform Agar CCA Agar
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₹ 7665 / Piece Get Latest Price
Product Brochure
| Packaging Size | 25 Kg |
| Packaging Details | Plastic Bottle |
| Final pH | 25 |
| Storage Temperature | 15-25 |
| Product Code | M1991I |
| Country of Origin | Made in India |
| Brand | Himedia |
| Form | Powder |
| Usage | Laboratory/Industry or R&D |
Minimum order quantity: 1 Piece
HiCrome™ Chromogenic Coliform Agar (CCA Agar) is a selective medium used to detect coliforms and Escherichia coli in water and food samples. It contains chromogenic substrates that detect the enzymes ß-galactosidase and ß-glucuronidase. What it detects:
- ColiformsProduce pink colonies due to the cleavage of one chromogen by the enzyme ß-galactosidase
- Escherichia coliProduce dark blue to violet colonies due to the cleavage of both chromogens by the enzymes ß-galactosidase and ß-glucuronidase
- The medium contains nutrients like peptone special and sodium pyruvate to help organisms grow
- Sodium lauryl sulfate inhibits gram-positive organisms
- L-Tryptophan improves the indole reaction, which increases detection reliability
- To confirm E. coli, add a drop of Kovacs reagent to the dark-blue to violet colony
- If the color turns cherry-red, the reaction is positive
- Store in a tightly closed container between 15-25°C
- Use before the expiry date on the label
- Dispose of used or unusable preparations by autoclaving or incineration
Buffered Sodium Chloride- Peptone Solution pH 7.0
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₹ 3765 / Kg Get Latest Price
Product Brochure
| Purity | 99% |
| Packaging Details | Plastic Bottle |
| Grade | Laboratory |
| Usage/Application | Laboratory |
| Form | Powder |
| Concentration | 25% |
| Country of Origin | Made in India |
| Brand | HIMEDIA |
| Packaging Size | 500gm |
| Product Code | MH1275-500G |
Minimum order quantity: 1 Kg
Buffered Sodium Chloride-Peptone Solution pH 7.0 is a diluent solution used in microbial limit testing, particularly in pharmaceutical and cosmetic industries. It helps maintain microbial viability, osmotic balance, and pH stability during sample preparation for testing. The solution is composed of peptone, sodium chloride, and phosphate buffers, which contribute to these properties. Key characteristics and uses:
- Diluent for Microbial Limit Testing:It's recommended as a diluent for microbial limit testing in accordance with harmonized methods (USP/EP/BP/JP/IP).
- Maintains Microbial Viability:The solution's composition helps maintain the viability of microorganisms during sample preparation and testing.
- Osmotic Stability:Sodium chloride in the solution helps maintain osmotic balance, preventing microbial lysis.
- pH Stability:The phosphate buffer helps maintain a stable pH of 7.0 ± 0.2, ensuring optimal conditions for microbial growth and testing.
- Nutrient Source:Peptone provides a nutrient source for microorganisms, although the low level of peptone is chosen to minimize physiological shock to the microorganisms when suspended in the diluent.
- Used in Various Industries:It is employed in pharmaceutical, cosmetic, food, water, and wastewater industries for microbial examination and enumeration.
- Harmonized Methodology:The composition of this solution aligns with the harmonized methodology of pharmaceutical products as described in USP/EP/BP/JP/IP.
Fluid Casein Digest Soya Lecithin Medium
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₹ 10945 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Form | Powder |
| Grade | LR (Laboratory) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory/Microbiology R&D |
| Color | White |
| Country of Origin | Made in India |
| Packing Size | 500G |
| Product Code | M117 |
Minimum order quantity: 1 Kg
Fluid Casein Digest Soya Lecithin Medium is a specialized microbiological culture medium used for cultivating and isolating a wide range of microorganisms, particularly those requiring complex organic compounds. It is frequently employed in microbial limit tests and sanitary surface examinations. The medium is formulated to neutralize certain disinfectants, making it suitable for assessing microbial contamination on surfaces treated with these agents. Key Features and Components:
- Nutrient-rich:It contains casein digest (or soy hydrolysate as an alternative) and soya lecithin, providing essential nutrients for microbial growth.
- Neutralizing agents:The presence of soya lecithin and polysorbate 20 (in some formulations) helps neutralize quaternary ammonium compounds and phenolic disinfectants, respectively.
- Versatile:Suitable for various applications, including clinical, environmental, and food microbiology.
- Microbial Limit Tests:Recommended by the United States Pharmacopeia (USP) for microbial limit tests.
- Sanitary Surface Examination:Used to assess the effectiveness of cleaning and disinfection procedures by detecting residual microorganisms on surfaces.
- Sanitary Examination of Surfaces:This medium is specifically recommended for assessing the microbial contamination of surfaces after cleaning and disinfection, especially those treated with quaternary ammonium compounds or phenolic disinfectants.
- Microbial Limit Tests:It is utilized in the pharmaceutical and food industries to determine the total viable count of microorganisms in various products and raw materials, as per USP and IP standards.
- Growth Promotion:It provides a rich nutrient base for the growth of diverse microorganisms, making it useful for cultivating a wide range of bacterial and fungal species.
- Environmental Monitoring:Used in the microbiological analysis of environmental samples, including those from food production facilities and other settings.
- Neutralization of Disinfectants:The medium's neutralizing agents (lecithin and polysorbate 20) allow for the recovery and detection of microorganisms that might otherwise be inhibited by disinfectants present on surfaces.
Dey Engley Neutralizing Broth
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₹ 6095 / Piece Get Latest Price
Product Brochure
| Grade | AR (Analytical) |
| Packaging Size | 500G |
| Brand | Himedia |
| Usage/Application | Laboratory/Pharmaceutical or R&D |
| Packaging Details | Plastic Bottle |
| Category | Himedia |
| Physical State | Powder |
| Country of Origin | Made in India |
| Make | Himedia |
| Packing Size | 500G |
| Packing Type | Plastic Bottle |
| Product Code | M1062 |
Minimum order quantity: 1 Piece
- Dey-Engley Neutralizing Broth is a specialized culture medium used to neutralize and test the effectiveness of antiseptics and disinfectants. It's designed to counteract a broad spectrum of antimicrobial agents, including quaternary ammonium compounds, phenolics, iodine and chlorine-based solutions, mercurials, formaldehyde, and glutaraldehyde. The broth contains neutralizing agents like lecithin, Tween 80, sodium thiosulfate, and sodium bisulfite, which bind to and deactivate these chemicals, allowing for accurate assessment of the antimicrobial agent's bactericidal activity. Bromocresol purple acts as a pH indicator, changing color upon dextrose fermentation, which can be used to confirm neutralization and bacterial growth. Here's a more detailed breakdown:Purpose:
- Neutralization:The primary function is to neutralize the effects of various antiseptics and disinfectants, preventing them from interfering with subsequent microbial growth.
- Disinfectant Testing:It's used in procedures to determine the effectiveness of disinfectants by assessing their ability to inhibit or kill microorganisms after neutralization.
- Neutralizing Agents:
- Lecithin: Neutralizes quaternary ammonium compounds and helps to neutralize ethanol.
- Tween 80 (Polysorbate 80): Neutralizes phenolic compounds and hexachlorophene.
- Sodium Thiosulfate and Sodium Bisulfite: Help neutralize chlorine-based disinfectants.
- Nutrients:Tryptone and yeast extract provide essential nutrients for microbial growth.
- Dextrose:A fermentable carbohydrate that serves as a substrate for microorganisms and, when fermented, produces acid, causing a color change in the medium.
- Bromocresol Purple:An indicator dye that changes from purple to yellow when dextrose is fermented by bacteria.
- 1. Neutralization:Antiseptic or disinfectant is added to the Dey-Engley Neutralizing Broth, and the neutralizing agents in the broth bind to and deactivate the antimicrobial agent.
- 2. Inoculation:A known concentration of microorganisms (bacteria or fungi) is added to the broth culture.
- 3. Incubation:The inoculated broth is incubated under appropriate conditions to allow for microbial growth.
- 4. Observation:The medium is observed for signs of microbial growth (e.g., turbidity, color change).
- 5. Interpretation:If the antiseptic/disinfectant is effective, microbial growth will be inhibited. If the antiseptic/disinfectant is neutralized, microbial growth will occur, and the color of the medium may change due to dextrose fermentation.
Cetrimide Agar Himedia 500ml
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₹ 4010 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| CAS Number | 1119 97 7 |
| Molecular Weight | 336.4 g/mol |
| Molecular Formula | C17H38BrN |
| Color | Yellow |
| Country of Origin | Made in India |
| Usage/Application | Laboratory/Pharmaceutical or R&D |
| CAS No. | 57-09-0 |
| Make | Himedia |
| Form | Powder |
| Product Code | MH024 |
| Appearance | Cream To Yellow Homogeneous Free Flowing Powder |
| PH | 7.00-7.40 |
| Storage | Store Between 10-30 DegreeC |
| Cultural Response | Cultural Response Cultural Characteristics Observed After Incubation At 30-35 Degreec For 18-72 Hour |
| Gelling | Firm, Comparable With 1.36% Agar Gel |
| Colour and Clarity of prepared medium | Light Amber Coloured Opalescent Gel With A Slight Precipitate Forms In Petri Plates |
Minimum order quantity: 1 Kg
Cetrimide agar is a selective and differential medium used to isolate and identify Pseudomonas aeruginosa from various samples. It contains cetrimide, a detergent that inhibits the growth of most bacteria except for P. aeruginosa and some other Pseudomonas species. The medium also enhances the production of pyocyanin, a blue-green pigment, and pyoverdin, a yellow-green fluorescent pigment, which are characteristic of P. aeruginosa. Key Features and Applications:
- Selective Medium:Cetrimide agar is selective because cetrimide inhibits the growth of many other bacteria, allowing P. aeruginosa to grow preferentially.
- Differential Medium:The medium can differentiate P. aeruginosa from other Pseudomonas species based on its ability to produce pyocyanin and pyoverdin.
- Isolation and Identification:It's used for the isolation of P. aeruginosa from various samples, including clinical specimens, environmental samples, and pharmaceutical products.
- Harmonized Method:The medium is used in microbial limit testing according to harmonized methodologies of USP/EP/BP/JP/IP.
- Pigment Production:P. aeruginosa grown on cetrimide agar produces pyocyanin (a blue, water-soluble pigment) and pyoverdin (a yellow-green fluorescent pigment).
- Pancreatic Digest of Gelatin: A source of nitrogen.
- Magnesium Chloride: Enhances pyocyanin production and neutralizes EDTA.
- Dipotassium Sulfate: Enhances pyocyanin production.
- Cetrimide: The selective agent that inhibits the growth of most bacteria.
- Agar: The solidifying agent.
- Glycerol: A source of carbon.
- 1. Dehydrated Form:Cetrimide agar is available in a dehydrated form, which is dissolved in distilled water and autoclaved.
- 2. Pouring Plates:The medium is cooled and poured into sterile Petri dishes.
- 3. Inoculation:Samples are inoculated onto the agar plates and incubated at appropriate temperatures (typically 37°C for clinical samples and 30-35°C for some other Pseudomonas species).
- 4. Identification:P. aeruginosa colonies are typically identified by their characteristic blue-green pigment production and, in some cases, a grape-like odor.
- P. aeruginosa is a common nosocomial pathogen and can cause various infections, including pneumonia, bacteremia, and urinary tract infections.
- The characteristic blue-green pigment (pyocyanin) and fluorescent pigment (pyoverdin) production help differentiate P. aeruginosa from other Pseudomonas species and other bacteria.
Soyabean HiVe Medium Sterile Powder Tryptone Soya HiVe Broth Sterile Powder)
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₹ 2905 / Kg Get Latest Price
Product Brochure
| Grade | AR (Analytical) |
| Packaging Size | 500 g |
| Brand | Himedia |
| Usage/Application | Laboratory |
| Packaging Details | Bottle |
| Physical State | Powder |
| Country of Origin | Made in India |
| Form | Powder |
| Packing Type | Bottle |
Minimum order quantity: 1 Kg
Soyabean HiVeg™ Medium (also known as Tryptone Soya HiVeg™ Broth) is a sterile powder medium used for the cultivation of a wide range of microorganisms, particularly in sterility testing and general microbial growth studies. It's a gamma-irradiated, sterile powder and is notable for using vegetable-based peptones instead of animal-derived ones, thus avoiding BSE/TSE/GMO risks. Key features and uses:
- Sterility Testing:It's commonly used in manufacturing processes to evaluate the presence of microorganisms, ensuring the sterility of products and processes.
- Microbial Cultivation:Supports the growth of various microorganisms, including aerobic, facultative, and anaerobic bacteria, as well as fungi.
- Vegetable Peptones:The medium is formulated with vegetable peptones to replace animal-derived peptones, addressing concerns about BSE/TSE/GMO contamination.
- Gamma-Irradiated:The medium is sterilized using gamma irradiation, ensuring a high level of sterility.
- Pharmacopeial Compliance:It is often specified in pharmacopoeias for various applications, including sterility testing and microbial limit tests.
- Sensitivity Testing:It can be used in the tube dilution method for testing the sensitivity of antimicrobial agents.
- Medium Color Change:A color change from purple to yellow with turbidity indicates microbial growth in some variations of the medium.
- Tryptone
- Soya Peptone
- Sodium Chloride
- Dextrose (or other carbohydrates)
- Dibasic Potassium Phosphate
- Other ingredients to adjust pH and performance
- Dissolve the sterile powder in distilled or deionized water.
- Heat if necessary to ensure complete dissolution.
- Dispense the medium into desired containers (tubes, plates, etc.).
- Sterilize by autoclaving.
- Dehydrated medium should be stored in a tightly closed container at temperatures below 30°C.
- Prepared medium should be refrigerated (2-8°C).
Sabouraud Dextrose Broth
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₹ 2780 / Piece Get Latest Price
Product Brochure
| Grade | AR (Analytical) |
| Packaging Size | 500 g |
| Brand | Himedia |
| Usage/Application | Laboratory |
| Packaging Details | Plastic Bottle |
| Packaging Type | Bottle |
| Physical State | Powder |
| Grade Standard | Analytical Grade |
| Country of Origin | Made in India |
| Solubility | 65.00 gm |
| Make | Himedia |
Minimum order quantity: 1 Piece
Sabouraud Dextrose Broth (SDB) is a liquid culture medium primarily used for cultivating and isolating yeasts, molds, and dermatophytes. Its high dextrose concentration and low pH (around 5.6) promote the growth of these microorganisms while inhibiting many bacterial species. SDB is a complex medium, meaning it contains multiple ingredients that provide nutrients for growth, including peptone (digested animal tissue and casein), dextrose (a sugar), and other vitamins and minerals. Here's a more detailed breakdown:
- Purpose:SDB is used in various applications, including:
- Cultivating and isolating yeasts, molds, and dermatophytes.
- Testing the antifungal activity of pharmaceutical products.
- Microbiological examination of non-sterile products, such as those in the pharmacopeia (USP, EP, JP).
- Detecting the presence of Candida albicans in non-sterile products.
- Key Components:
- Peptone: Provides amino acids, nitrogen, carbon, vitamins, minerals, and growth factors.
- Dextrose: Serves as a carbohydrate source and energy for microbial growth. The high dextrose concentration inhibits bacterial growth.
- Low pH: The acidic pH (around 5.6) favors the growth of fungi and inhibits many bacteria.
- Appearance and Other Characteristics:
- SDB is typically a clear, light amber-colored liquid.
- The pH is usually adjusted to 5.6 ± 0.2 at 25°C.
- It is a complex medium, meaning it contains various ingredients that provide the necessary nutrients for fungal growth.
- Growth Promotion:SDB promotes the formation of spores (conidia and sporangia) and pigments in yeasts and molds.
- Preparation:
- SDB is often available as a dehydrated powder that needs to be dissolved in distilled or purified water.
- The specific instructions for preparation may vary depending on the manufacturer and the specific type of SDB (e.g., with chloramphenicol).
Violet Red Bile Agar Himedia
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₹ 4030 / Piece Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | HIMEDIA |
| Packaging Details | Plastic Bottle |
| Prescription/Non prescription | Prescription |
| Usage/Application | Laboratory |
| Product Code | M049-500G |
| Form | Powder |
| Shelf Life | 36 Months |
| Country of Origin | Made in India |
Minimum order quantity: 500 Piece
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- HiMedia Violet Red Bile Agar is a selective microbiological culture medium used to isolate and count coliform bacteria in food, water, and clinical samples. It suppresses the growth of non-coliform bacteria, while the lactose and neutral red indicator system differentiates lactose-fermenting coliforms, which appear as red to pink colonies surrounded by a reddish precipitate. A modified version, HiCynth™ Agar, uses chemically defined peptones to eliminate animal-based protein risks.
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Plate Count Agar Standard Methods Agar
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₹ 4365 / Piece Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Bottle |
| Material | Plastic |
| Usage/Application | Laboratory |
| Color | White |
| Country of Origin | Made in India |
| Shelf Life | 36 Months |
| HSN Code | 38210000 |
| Colour | Light biege |
| Form | Powder |
| Make | Himedia |
| Product Code | M091 |
Minimum order quantity: 1 Piece
Plate Count Agar (PCA), also known as Standard Methods Agar, is a non-selective, general-purpose microbiological growth medium used to determine the number of viable microorganisms (primarily bacteria) in a sample. It's commonly used to assess the total microbial load in foods, water, and other materials of sanitary importance. Here's a more detailed breakdown:Purpose and Usage:
- Enumeration of Microorganisms: PCA is used to count the number of viable bacteria, yeasts, and molds in a sample, typically by the pour plate or spread plate method.
- Quality Control: It's a standard tool for monitoring microbial contamination in various industries, including food production, water treatment, and clinical settings.
- Testing: PCA is used in testing for microbial contamination in food and water.
- Non-selective:PCA doesn't contain any ingredients that inhibit or favor the growth of specific microorganisms. This means it allows a wide range of microorganisms to grow, providing a general count of the viable microbial population.
- Nutrient-rich:The medium contains nutrients like peptone, yeast extract, and dextrose, which provide the necessary carbon and nitrogen sources for microbial growth.
- Standard Formulation:The formula is based on the Buchbinder et al. method, which is recommended by the American Public Health Association (APHA) and the Food and Drug Administration (FDA) for determining plate counts.
- 1. Sample Preparation:A sample (e.g., food, water) is diluted using sterile saline to obtain a manageable number of microorganisms for plating.
- 2. Plating:The diluted sample is then plated onto PCA plates. This can be done by either the pour plate method (where molten agar is mixed with the sample) or the spread plate method (where a small amount of the sample is spread on the surface of solidified agar).
- 3. Incubation:The plates are incubated at a specific temperature (typically 32-37°C for bacteria) for a set period (usually 24-48 hours).
- 4. Colony Counting:After incubation, individual colonies (each representing a single microbial cell or group of cells) are counted on the plates.
- 5. Interpretation:The number of colonies is used to calculate the number of microorganisms per unit volume or weight of the original sample.
Macconkey Agar Himedia
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₹ 3550 / Kg Get Latest Price
Product Brochure
| Grade | AR (Analytical) |
| Packaging Size | 500gm |
| Packaging Type | Bottle |
| Category | Complex Media |
| Physical State | Powder |
| Brand | HIMEDIA |
| Product Code | M008-500G |
Minimum order quantity: 1 Kg
A modification of the original medium with the exception of crystal violet, selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens. Recommended for use in microbiological examination of foodstuffs and for direct plating / inoculation of water samples for coliform counts . These media are also accepted by the Standard Methods for the Examination of Milk and Dairy Products. HiMedia M008 refers to MacConkey Agar, a selective & differential microbiological medium used to isolate and differentiate lactose fermenting (pink/red colonies) from non-lactose fermenting (colorless) Gram-negative enteric bacteria, crucial for identifying pathogens like E. coli, Salmonella, and Shigella from clinical samples, appearing as a light yellow to pink powder that forms a red/orange gel, inhibiting Gram-positive growth via bile salts and crystal violet. Key Features & Use
- Type: Selective & Differential Agar.
- Purpose: Isolates Gram-negative bacteria; differentiates lactose fermenters (pink/red) from non-fermenters (colorless).
- Components: Contains peptone, bile salts, lactose, neutral red (pH indicator), and agar. Crystal violet (CV) may be included or excluded depending on the specific variant (e.g., M008 vs. M081).
- Mechanism: Bile salts and crystal violet inhibit Gram-positive bacteria; lactose & neutral red differentiate based on fermentation, turning colonies pink/red when acid is produced.
- Appearance: Light yellow to pink powder; forms a red/orange gel when prepared.
- Applications: Culturing enteric bacteria from clinical samples, food, and water.
- E. coli, Klebsiella: Pink to red colonies with bile precipitate (lactose fermenters).
- Salmonella, Shigella: Colorless, transparent colonies (non-lactose fermenters).
- Staphylococcus aureus: Inhibited (Gram-positive).
Nutrient Agar Himedia
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₹ 3635 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Manufacturer | HIMEDIA |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Composition | Peptone,Sodium Chloride,Yeast Extract ,Agar |
| Prescription/Non prescription | Prescription |
| Final pH | 7.60 pH |
| Usage/Application | Laboratory/R&D |
| Storage Temperature | 25 Degree C |
| Product Code | M001 |
| Country of Origin | Made in India |
| Pack Size | 500G |
Minimum order quantity: 1 Bottle
Nutrient Agar, manufactured by HiMedia, is a general-purpose culture medium used for the cultivation and enumeration of microorganisms, particularly non-fastidious bacteria. It's a widely used medium for maintaining cultures, purity checks, and as a base for enriched media when supplemented with blood or other biological fluids. Key Features and Uses:
- General-purpose medium:Nutrient Agar is suitable for growing a broad range of bacteria, yeasts, and molds.
- Cultivation and Enumeration:It's used to cultivate microorganisms and to determine the number of viable organisms in a sample.
- Enrichment:Nutrient Agar can be supplemented with blood, serum, or other biological fluids to support the growth of more fastidious organisms.
- Purity Checks:It's useful for ensuring the purity of bacterial cultures before further biochemical or serological testing.
- Maintenance of Cultures:Nutrient Agar can be used to maintain bacterial cultures for extended periods.
- Peptone: Provides a source of nitrogen, vitamins, and other essential nutrients.
- Beef extract/Yeast extract: Adds water-soluble nutrients like vitamins, carbohydrates, and salts.
- Sodium chloride: Maintains osmotic balance.
- Agar: A solidifying agent that provides a solid surface for bacterial growth.
- Standard Nutrient Agar No. 1 (M1210): Formulated according to APHA standards for cultivation and enumeration of non-fastidious microorganisms.
- Nutrient Agar (M001): A general-purpose medium for cultivating and enumerating microorganisms.
- Nutrient Agar No.2 (M1269/M1269S): Used for microbiological analysis of water and other applications.
- Nutrient Agar pH 6.8 (M561): A variation of Nutrient Agar with a specific pH.
- Nutrient Agar pH 7.0 (M561A): Another variation with a different pH.
- Nutrient Agar Medium (MM012): Suitable for microbial limit testing according to the Indian Pharmacopoeia.
Fluid Thioglycollate Medium Thioglycollate Medium Fluid
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₹ 1965 / Bottle Get Latest Price
Product Brochure
| Grade | AR (Analytical) |
| Packaging Size | 500gm |
| Usage/Application | Laboratory/Pharmaceuticals or R&D |
| Packaging Details | Plastic Bottle |
| Packaging Type | Bottle |
| Physical State | Powder |
| Purity | 98% |
| Shelf Life | 36 Months |
| Country of Origin | Made in India |
| Brand | HIMEDIA |
| Product Code | M009-500G |
Minimum order quantity: 1 Bottle
Fluid Thioglycollate Medium (FTM) is a liquid culture medium used for growing both aerobic and anaerobic microorganisms, particularly in sterility testing. It's often packaged in plastic bottles and contains ingredients like peptone, yeast extract, glucose, L-cystine, sodium thioglycollate, and a small amount of agar. The medium's pink ring in suspension and pH of 7.1 ± 0.2 helps to identify different types of microorganisms. Key Features and Uses:
- Sterility Testing:FTM is widely used for testing the sterility of biological products, pharmaceuticals, and other materials, as it can support the growth of both aerobes and anaerobes.
- Cultivation:It's a general-purpose medium for cultivating a variety of microorganisms, including fastidious anaerobes and aerobes.
- Oxygen Tolerance:FTM's unique composition allows for the determination of the oxygen requirements of different bacteria.
- Composition:The medium contains a reducing agent (L-cystine and sodium thioglycollate) and a small amount of agar to help maintain anaerobic conditions in the lower part of the bottle.
- Indicator:The presence of resazurin dye helps to indicate the oxygen level in the medium, with the lower, yellowish part indicating anaerobic conditions and the pink ring in suspension indicating oxygen.
- Plastic Bottles: FTM is commonly packaged in plastic bottles, often with screw caps and septa for ease of access and inoculation.
- Double Packing: Some manufacturers offer double-packed bottles for enhanced sterility and ease of cleaning procedures.
- Storage: The medium is typically stored at 2-25°C.
- Incubation Temperature: The incubation temperature usually ranges from 30-35°C.
- Incubation Time: Incubation time can vary, but typically ranges from 7-14 days.
Mannitol Salt Agar 500g
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₹ 3670 / Gram Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Final pH | 7.20 - 7.60 |
| Usage/Application | Laboratory |
| Product Code | MH118 |
| CAS Number | 143-74-8 |
| Country of Origin | Made in India |
| Shelf Life | 36 MONTHS |
| Product Name | Mannitol Salt Agar |
| Form | Powder |
| Make | Himedia |
Minimum order quantity: 1 Gram
Mannitol Salt Agar (MSA) is a type of growth medium used in microbiology that is both selective and differential. It's primarily used to isolate and identify Staphylococcus aureus from other bacteria, especially those that can tolerate high salt concentrations. MSA contains a high salt concentration (7.5%), which inhibits the growth of most bacteria, making it selective for halotolerant organisms like staphylococci. It also differentiates Staphylococcus aureus from other staphylococci based on their ability to ferment mannitol, a sugar alcohol, which is indicated by a color change in the medium. Here's a more detailed explanation:
- Selective:MSA selects for Staphylococcus species (and other halotolerant bacteria) due to its high salt concentration (7.5% NaCl). This inhibits the growth of most Gram-negative bacteria and many Gram-positive bacteria except those that can tolerate high salt.
- Differential:MSA distinguishes between Staphylococcus aureus and other staphylococci based on their ability to ferment mannitol. Staphylococcus aureus can ferment mannitol, producing acidic byproducts that lower the pH of the medium, causing the pH indicator phenol red to turn yellow. Other staphylococci that cannot ferment mannitol will grow with pink or red colonies.
- Other Components:Besides NaCl, MSA also contains beef extract and proteose peptone, which provide essential nutrients for bacterial growth.
- Purpose:MSA is commonly used in labs to identify presumptive Staphylococcus aureus isolates, particularly in clinical samples or food samples.
- Growth on MSA:
- Staphylococcus aureus colonies will appear yellow or with a yellow halo due to mannitol fermentation.
- Other staphylococci will appear pink or red on the medium.
- Most other bacteria are inhibited by the high salt concentration.
Soybean Casein Digest Agar
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₹ 4955 / Gram Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | HiMedia |
| Packaging Details | Plastic Bottle |
| Product Code | MH290 |
| Country of Origin | Made in India |
| Form | Powder |
| Make | Himedia |
| Usage | Laboratory/Microbiological or R&D |
Minimum order quantity: 1 Gram
Casein-Soyabean Digest Agar (SCDA) is a general-purpose medium used to grow a wide variety of microorganisms. It's also known as Tryptone Soya Agar. What it's used for?
- Microbial limit tests: Used to test for microbial contamination in products like cosmetics
- Antimicrobial preservative tests: Used to test the effectiveness of antimicrobial preservatives
- Sterility testing: Used to test for sterility in products
- Maintenance of stock cultures: Used to maintain cultures of microorganisms
- Phage and colicin typing: Used to type phages and colicins
- Haemophilus differentiation: Used to determine if Haemophilus isolates require X and V growth factors
- Production of antigens and toxins: Used to produce antigens and toxins in the health industry
- Detection of antimicrobial agents: Used to detect antimicrobial agents in food and other products
- The amino acids and long chain peptides in the medium provide nutrients for microorganisms
- Sodium chloride maintains the osmotic balance in the medium
Peptone Bacteriological Himedia
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₹ 3085 / Kg Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory/Microbiology or R&D |
| Color | Light yellow |
| Country of Origin | Made in India |
| Form | Powder |
| Product Code | RM001 |
| CAS No | 73049-73-7 |
| Odour | Characteristic Odour But Not Putrescent |
Minimum order quantity: 1 Kg
HiMedia Bacteriological Peptone is a nutrient-rich powder derived from the enzymatic digestion of animal tissues. It's used as a media ingredient in microbiology for cultivating a wide range of microorganisms, including bacteria, fungi, and other microbes. It provides essential nutrients like nitrogen and amino acids, supporting microbial growth in various applications like fermentation, vaccine production, and antibiotic manufacturing. Key Features and Characteristics:
- Nutrient Source: It acts as a source of nitrogen and amino acids, crucial for microbial growth and metabolism.
- Wide Application: Employed in various microbiological settings, including media for bacterial cultivation, fermentation processes, and the production of enzymes, vaccines, and antibiotics.
- Composition: Primarily composed of amino acids derived from the enzymatic digestion of meat.
- Appearance: A light yellow to brownish-yellow, free-flowing powder.
- Solubility: Freely soluble in distilled or purified water.
- Clarity: A 2% w/v aqueous solution should remain clear and neutral after autoclaving.
- Cultural Response: It supports good growth of various microorganisms when incorporated into culture media.
- Storage: Recommended to store below 30°C.
- Controlled Conditions:HiMedia Bacteriological Peptone is prepared under controlled conditions to ensure quality and consistency.
- High Tryptophan Content:It's notable for its high tryptophan content, which can be important for certain biochemical tests.
- Nutritional Benefits:It is known to provide nutritional benefits, especially at low dilution rates, which makes it useful for recombinant cell line cultures.
Xylose Lysine Deoxycholate Agar XLD Agar
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₹ 4355 / Bottle Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Molecular Weight | 146.19 g/mol |
| Chemical Formula | C6H14N2O2 |
| Country of Origin | Made in India |
| Physical state | Solid |
| Shelf life | 36 Months |
| Form | Powder |
| Pack Size | 500G |
| Pack Type | Plastic Bottle |
| Brand Name | HIMIEDA |
| Usages | Laboratory |
| Colour | Red coloured medium |
Minimum order quantity: 1 Bottle
Xylose Lysine Deoxycholate Agar (XLD Agar) is a selective and differential medium used for isolating and identifying Salmonella and Shigella bacteria, particularly from clinical samples and food. It's characterized by its pink color, a free-flowing, homogeneous powder. XLD Agar relies on sodium deoxycholate as a selective agent to inhibit Gram-positive microorganisms, and a combination of xylose, lysine, and other components for differential growth and identification of specific bacteria. Here's a more detailed breakdown:Selective Features:
- Sodium Deoxycholate: This component inhibits the growth of most Gram-positive bacteria, ensuring that only Gram-negative enteric bacteria like Salmonella and Shigella can thrive.
- Xylose:Salmonella and Shigella utilize xylose, a sugar, as a carbon source, leading to the production of acid, which changes the pH indicator (phenol red) from red to yellow, allowing for differentiation.
- Lysine:Lysine is used as a carbon and nitrogen source. After xylose is exhausted, Salmonella will decarboxylate lysine, producing an alkaline pH, which mimics the Shigella reaction and can help distinguish them.
- Hydrogen Sulfide Production:Some Salmonella species can produce hydrogen sulfide (H2S) from the thiosulfate in the medium, resulting in black colonies, a characteristic feature for differentiation.
- Lactose, Sucrose, and Phenol Red:These components, along with lysine and xylose, are part of the differential system, allowing for the identification of bacteria based on their fermentation patterns and hydrogen sulfide production.
- Salmonella: Red to pink colonies, some with black centers due to hydrogen sulfide production.
- Shigella: Red colonies, as they do not ferment xylose or produce hydrogen sulfide.
- Coliform Bacteria (e.g., E. coli): Yellow or orange colonies.
- Isolation of Salmonella and Shigella:XLD Agar is used to isolate these bacteria from various sources, including stool samples, food, and clinical specimens.
- Identification of Enteric Pathogens:It helps differentiate between different enteric pathogens, including Salmonella, Shigella, and other Gram-negative bacteria.
- Pharmaceutical and Food Testing:XLD Agar is also used in pharmaceutical and food testing to detect the presence of Salmonella and other bacteria.
Sabouraud Dextrose Agar Himedia
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₹ 3785 / Gram Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | HIMEDIA |
| Packaging Details | MH063 |
| Prescription/Non prescription | Prescription |
| Usage/Application | Laboratory/Pharmaceutical or R&D |
| Product Code | MH063 |
| Form | Powder |
| Country of Origin | Made in India |
| pH | 5.4-5.6 |
Minimum order quantity: 500 Gram
Sabouraud Dextrose Agar (SDA) from HiMedia is a specialized media for cultivating fungi, particularly yeasts and molds. It's designed to inhibit bacterial growth while promoting the growth of fungi due to its acidic pH and high dextrose concentration. Here's a more detailed description:
- Purpose:SDA is primarily used for the isolation and cultivation of fungi, including yeasts and molds, from various sources, including clinical specimens, food, and environmental samples.
- Composition:It typically contains dextrose (a sugar), peptone (a source of nitrogenous compounds and nutrients), and agar (a solidifying agent).
- pH:The medium has an acidic pH (around 5.4-5.6), which is inhibitory to many bacteria.
- Growth Conditions:The high concentration of dextrose and acidic pH favor the growth of fungi while inhibiting bacterial growth.
- Applications:
- Isolation of fungi, especially those associated with skin infections.
- Determination of total yeast and mold counts (TYMC) in environmental monitoring.
- Detection of fungi in dietary supplements.
- Subculture of Candida albicans.
- Formulations:HiMedia offers SDA in various forms, including granulated, ready-to-use plates, and slants.
- Storage:It's crucial to store SDA according to the manufacturer's instructions to maintain its performance and stability.
- Selective Media:SDA can be used as a selective medium by adding inhibitors to suppress bacterial growth. For example, chloramphenicol is often added to inhibit gram-negative and gram-positive bacteria.
Reinforced Medium for Clostridia
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₹ 4990 / Piece Get Latest Price
Product Brochure
| Packaging Size | 500 g |
| Grade | AR (Analytical) |
| Brand | Himedia |
| Packaging Details | Plastic Bottle |
| Usage/Application | Laboratory |
| Material | Plastic |
| Color | Yellow |
| Country of Origin | Made in India |
| pH-value | 6.6 - 7.0 |
| CAS No | 52-89-1 |
| Product Code | MH443 |
Minimum order quantity: 1 Piece
Reinforced Clostridial Medium (RCM) is a non-selective, nutritious broth used for the cultivation, isolation, and enumeration of Clostridium spp. and other anaerobes. It's highly nutritious, supporting growth from small inocula and achieving the highest viable count of Clostridia. RCM can be used to enrich growth broths before subculturing clostridia to solid agar. Here's a more detailed breakdown:
- Purpose:RCM is used to cultivate and enumerate Clostridium spp. and other anaerobes in various applications, including food and pharmaceutical industries.
- Nutrient Source:The medium is rich in nutrients like amino acids, peptides, carbohydrates, and vitamins, provided by ingredients like beef extract and casein peptone.
- Energy Source:Dextrose provides a carbohydrate energy source for bacterial growth.
- Reducing Agent:L-cysteine HCl acts as a reducing agent, creating anaerobic conditions suitable for Clostridium growth.
- Other Ingredients:Sodium chloride maintains osmotic equilibrium, and low concentrations of soluble starch help detoxify metabolic byproducts.
- Applications:
- Food Industry: Used for the growth and enumeration of spores in dairy products, canned foods, and other food items.
- Pharmaceutical Industry: Used in microbial limit testing of pharmaceutical products in accordance with USP/EP/BP/JP/IP methodologies.
- Water Analysis: Used for the cultivation and enumeration of Clostridia from water samples.
- Variants:
- Differential Reinforced Clostridial Agar (DRCM): Used for the enumeration and cultivation of Clostridia from water, incorporating an indicator system for sulphite reduction (black colonies).
- Reinforced Clostridial Broth w/o Agar: A broth version of the medium without agar.
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