Nutrient Broth

Product Details:

HiMedia's Brain Heart Infusion (BHI) Broth is a highly nutritious, well-buffered liquid medium designed for the growth of a wide variety of microorganisms, including fastidious organisms and those associated with blood cultures. It serves as a general-purpose medium, is used for preparing inocula for antimicrobial testing, and supports the growth of bacteria, fungi, and yeasts. Its composition provides essential nutrients and electrolytes to support microbial growth, and it can be modified with additives like sodium chloride for selective isolation or sheep blood for fungal cultivation.
 Key Features and Uses:

• Highly Nutritious:Contains brain and heart infusions and peptones, providing essential proteins and nutrients to support microbial growth. 
• Supports Fastidious Organisms:Excellent for cultivating pathogenic cocci and other organisms with specific growth requirements, often used in blood culture work. 
• General-Purpose Medium:Suitable for the growth of a broad range of microorganisms, including bacteria, yeasts, and molds. 
• Inocula Preparation:Used to prepare bacterial inocula for antimicrobial susceptibility testing. 
• Selective Isolation:Can be adapted with additives for specific isolation. For example, adding 6.5% sodium chloride makes it selective for salt-tolerant bacteria like enterococci. 
• Fungal Cultivation:With the addition of defibrinated sheep blood, it is recommended for the isolation and cultivation of fungi such as Histoplasma capsulatum. 
• ISO Recommendation:The ISO committee has recommended this medium for detecting Staphylococcus aureus. 

Additional Information:

• Delivery Time: Ready Stock
• Packaging Details: Box

Product Details:

Rappaport Vassiliadis (RV) Medium by HiMedia is a selective enrichment broth used for isolating Salmonella species from various samples like food, environmental sources, and clinical specimens. It works by creating conditions that are toxic to most bacteria, such as low pH, high osmotic pressure from magnesium chloride, and the presence of malachite green, while allowing Salmonella to thrive. The medium is also used for testing pharmaceutical products according to pharmacopeia guidelines. Key features and description

• Purpose: To selectively enrich and promote the growth of Salmonella while inhibiting the growth of other bacteria.
• Composition: It contains ingredients that create a selective environment:
  • Soya peptone: Provides essential nutrients.
  • Magnesium chloride: Increases osmotic pressure.
  • Malachite green: Inhibits the growth of most other bacteria, especially gram-positive ones.
  • • Potassium and sodium phosphates: Buffer the medium to maintain a stable pH.
    • Sodium chloride: Helps maintain osmotic balance.
  • Incubation: The standard incubation temperature is 42−43∘C42 minus 43 raised to the composed with power cap C42−43∘𝐶.
  • Procedure: After incubation, the enriched culture is subcultured onto a selective agar medium, such as MacConkey Agar or XLD Agar, for isolation and identification.
  • Applications:
    • Enrichment of Salmonella from food and environmental samples.
    • Isolation of Salmonella from clinical specimens, including feces.
    • Testing pharmaceutical products according to USP/EP/BP/JP harmonized methodologies. 

Additional Information:

• Delivery Time: Ready Stock
• Packaging Details: Box