Leading Manufacturer of u40 supplement himedia, vancomycin ezy mi strip, netillin netilmicin sulphate himedia and thermo fisher scientific india pvt ltd from Jaipur.
U40 supplement from HiMedia is a filter-sterilized urea solution (5 ml per vial) used to detect urease activity in microbiological media. It's specifically recommended for use with Urea Agar Base (Christensen) and is a key component in identifying urease-positive organisms, particularly Proteus vulgaris. The supplement is a slight modification of the Christensen formulation and is recognized by the Bureau of Indian Standards (BIS). Here's a more detailed breakdown:
Function:U40 supplement is used to detect urease activity, which is the ability of an organism to produce the enzyme urease, which breaks down urea.
Composition:Each vial contains a urea solution, sufficient for 100 ml of medium.
Application:It's commonly used with Urea Agar Base (Christensen) for identifying bacteria, especially Proteus vulgaris, which is a rapid urease producer.
Benefits:The supplement helps differentiate between various urease-positive organisms, including those from the Enterobacteriaceae family, and those that are not.
Standards:The supplement's use is also recommended by the Bureau of Indian Standards under the specifications IS:5887(Part I)-1976.
References:Several publications, including those by Christensen, MacFaddin, and the Bureau of Indian Standards, are referenced in its application.
A Vancomycin Ezy MIC™ strip, from HiMedia, is a porous paper strip used for quantitative antimicrobial susceptibility testing to determine the minimum inhibitory concentration (MIC) of vancomycin against bacteria. It features a predefined concentration gradient of the antibiotic, printed scales for reading the MIC value in mcg/ml, and an identification symbol, allowing for the accurate measurement of bacterial growth inhibition on an agar plate. Key Features
Porous Paper Strip:Unlike traditional plastic strips, the Ezy MIC™ strip is made of porous paper, which adheres to the agar surface without forming air bubbles, ensuring accurate results.
Concentration Gradient:The strip is coated with vancomycin in a concentration gradient, meaning the antibiotic concentration decreases along the strip.
MIC Reading Scale:Both sides of the strip are printed with a scale to help read the MIC in micrograms per milliliter (µg/ml).
Identification Symbol:A three-letter symbol, "VAN", is printed on the strip to clearly identify it as a vancomycin strip.
Quantitative Measurement:The strip provides a quantitative method to determine the MIC, which is the lowest concentration of the antibiotic that inhibits bacterial growth.
How It Works
1. Preparation:The strip is placed on a bacterial culture grown on an appropriate agar medium, such as Mueller-Hinton Agar.
2. Inoculum Preparation:Bacteria are suspended in a broth to achieve a turbidity equivalent to a 0.5 McFarland standard, and then spread on the agar plate.
3. Incubation:The plate is incubated overnight to allow bacterial growth.
4. Reading the Result:After incubation, the zone of inhibition around the strip is observed. The MIC is determined by finding the point on the strip where the zone of inhibition ends.
Filter paper discs of 6 mm diameter with printed symbol and concentration of antibiotic o
Diameter
6mm
Netillin (Netilmicin Sulphate) from HiMedia is a disc containing the antibiotic Netilmicin Sulfate for Antimicrobial Susceptibility Testing (AST), which determines a bacterium's sensitivity to the drug. Netilmicin is a semisynthetic aminoglycoside antibiotic that inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, and it is used for treating serious Gram-negative infections, especially those resistant to gentamicin. Product Overview:
Product: Netillin (Netilmicin Sulphate)
Manufacturer: HiMedia Laboratories
Purpose: To be used as a reagent for antimicrobial susceptibility testing (AST).
Formulation: Impregnated onto a filter paper disc of a specific concentration.
How it Works (Mechanism of Action):
Netilmicin is an aminoglycoside antibiotic that works by interfering with bacterial protein synthesis.
It binds to the 30S ribosomal subunit of bacteria, preventing the proper binding of messenger RNA (mRNA) and interfering with the function of the acceptor tRNA site.
This leads to the inhibition of essential proteins needed for bacterial survival and growth.
Clinical Use of Netilmicin:
Treatment of Serious Infections:Netilmicin is used to treat severe infections, particularly those caused by Gram-negative bacteria that are resistant to other antibiotics like gentamicin.
Specific Infections:It is indicated for respiratory tract infections, urinary tract infections, and skin and soft tissue infections.
Method of Administration:Because it cannot be absorbed from the gut, netilmicin is administered by injection.
