Qualigens Safranine Staining Powder is a high-quality biological stain, a red cationic dye used for microscopy and staining, most commonly as a counterstain in the Gram stain procedure to color Gram-negative bacteria pink to red. It also stains bacterial spores and plant nuclei, and is useful for demonstrating glycosaminoglycans (GAGs) in cartilage by imparting an intense red color to them. The powder is a solid, brownish-red to dark brown substance, soluble in water and ethanol, and is typically a key reagent in histology, cytology, and botanical studies for visualizing tissue and cellular structures. Key characteristics and uses:
Chemical Nature: Safranin O (also known as Basic Red 2) is a cationic (positively charged) dye.
Color: It imparts an intense red color, making it a visible stain for various biological components.
Staining Applications:
Gram Staining: Used as a counterstain to identify Gram-negative bacteria, which appear pink to red.
Plant Anatomy: Stains plant nuclei and is used in conjunction with other stains like Safranine-fast green FCF for plant cells.
Cartilage and GAGs: Specifically binds to polyanions like proteoglycans (a type of GAG) in cartilage, staining them intensely red and helping to assess their depletion.
Bacterial Spores: Can be used for staining bacterial spores.
Physical Form: It is supplied as a solid powder, with a brownish-red to dark brown appearance.
Solubility: The powder is soluble in water and ethanol, allowing for the preparation of staining solutions.
Quality: Qualigens Safranine powder is a premium reagent designed for precise and reliable staining in biological research and laboratory applications.
Qualigens Eosin Yellow Stain Powder is a xanthene-derivative acid dye used in histology and microscopy, often as part of the H&E stain, to color cellular cytoplasm and connective tissue red with a yellowish hue, and also functions as a pH indicator. The powder is a fine yellow powder that, when dissolved, provides vibrant color to biological samples, aiding in visualization and analysis. Key characteristics and uses:
Appearance: A fine, yellow powder.
Chemical Type: An acid dye, a xanthene derivative of fluorescein, and an organobromine compound.
Primary Use: Staining biological samples in histology and microscopy, particularly for coloring cytoplasm and connective tissue.
Application in H&E Stain: Commonly used as the "E" in the popular Hematoxylin and Eosin (H&E) stain.
pH Indicator: Can act as a pH indicator, appearing yellow in acidic solutions and transitioning to red in alkaline conditions.
Other Applications: Also used in the production of inks and as a fluorescent dye in specialized microscopy.
Color Characteristic: Provides a red color with a somewhat yellowish cast to stained tissues.
