HiMedia's Barritt Reagent A is a light yellow colored solution used with Barritt Reagent B in the Voges-Proskauer (VP) test to detect acetoin, a neutral end product of glucose fermentation by bacteria. A positive VP test, indicated by the formation of a cherry-red color after adding both Barritt reagents, helps identify bacteria like Enterobacter aerogenes. This reagent is a for in-vitro use, stored at 15-25°C, and should not be exposed to skin or eyes. Purpose and Use
Voges-Proskauer Test: Barritt Reagent A is part of a two-reagent system used in the VP test.
Acetoin Detection: The test identifies bacteria that ferment glucose to produce neutral, non-acidic end products, primarily acetoin (acetyl methyl carbinol).
Bacterial Identification: The VP test is particularly useful for identifying members of the Enterobacteriaceae family.
Procedure
Inoculate a bacterial culture on MR-VP medium and incubate it for 24-48 hours.
Add Barritt Reagent A, followed by Barritt Reagent B (a potassium hydroxide solution).
Observe for the development of a cherry-red color within 5-10 minutes.
Interpretation of Results
Positive Test: A cherry-red color indicates the presence of acetoin and a positive VP test.
Negative Test: No red color formation signifies a negative VP test.
Storage and Safety
Storage: Store Barritt Reagent A at 15-25°C.
Safety: It is a combustible reagent and should be handled with care. Use appropriate personal protective equipment, such as gloves and eye protection, during handling.
For Laboratory Use: This reagent is intended for in-vitro diagnostic and professional use only.
